The validation and standardization of fluorescence methods is still in its infancy as compared to other prominent analytical and bioanalytical methods. Appropriate quality assurance standards are however a prerequisite for applications in highly regulated fields such as medical diagnostics, drug development, or food analysis.

For the first time, a team of recognized international experts has documented the present status of quality assurance in fluorescence measurements, and outlines concepts for establishing standards in this field.

This second of two volumes covers applications of fluorescence for bioanalysis and biomedical diagnostics.

For the first time, the present state of quality assurance and the need for establishing standards in fluorescence measurements are highlight here Concepts for establishing these standards particularly for highly regulated fields such as medical diagnostics, drug development, or food control are presented This second of two volumes covers fluorescence applications in bioanalysis and biomedical diagnostics Includes supplementary material: sn.pub/extras

Zusammenfassung

Analytical chemists and materials scientists will find this a useful addition to their armory. The contributors have sought to highlight the present state of affairs in the validation and quality assurance of fluorescence measurements, as well as the need for future standards. Methods included range from steady-state fluorometry and microfluorometry, microscopy, and micro-array technology, to time-resolved fluorescence and fluorescence depolarization imaging techniques.

Inhalt
Fluorescence Microscopy.- Need for Standardization of Fluorescence Measurements from the Instrument Manufacturer's View.- Characterization and Calibration in Wide Field and Sectioned Fluorescence Microscopy SIPcharts.- Quantitative Fluorescence Microscopy: Considerations and Controls.- Comparability of Fluorescence Microscopy Data and Need for Instrument Characterization of Spectral Scanning Microscopes.- Fluorescence Lifetime Imaging Microscopy: Quality Assessment and Standards.- Single Molecule Spectroscopy.- State of the Art and Novel Trends in Fluorescence Correlation Spectroscopy.- Single Molecule Spectroscopy: Instrumentation and Multiparameter Detection.- Fluorescence-Based Microarray Technology: Applications, Future Trends, and Need for Standardization.- DNA Microarrays: Applications, Future Trends, and the Need for Standardization.- Comparability of Microarray Experiments from the Instrument and the Sample Site and Approaches Towards Standardization.- Microarray Technology: Unresolved Issues and Future Challenges from a Regulatory Perspective.- Protein Arrays and Fluorescence Detection: Applications and Limitations.- Flow Cytometry.- Flow Cytometry: Instrumentation, Applications, Future Trends and Limitations.- Flow Cytometry Quality Assurance.- Approaches to Quantitation in Flow Cytometry.- Fluorescence Immunoassays.- Immunoassays: Basic Concepts, Physical Chemistry and Validation.- Time-Resolved Fluorometric Immunoassays; Instrumentation, Applications, Unresolved Issues and Future Trends.- Particle-Based Assays: Applications and Unresolved Issues.- Advances in Fluorescence Enzyme Detection Methods.- Quantitative PCR.- Quantitative Real-Time PCR: Fluorescent Probe Options and Issues.- Fluorescence In Situ Hybridization and Immunohistochemistry.- CellularBioimaging in Fluorescent Cancer Biomarker Evaluation: Validation, Technologies and Standards Development.- Fluorescence Technologies in Biomedical Diagnostics.- Fluorescence Techniques in Biomedical Diagnostics: Instrumentation, Analysis and Unresolved Issues.- In-vivo Fluorescence Imaging: Applications, Future Trends & Approaches to Standardization.