This volume provides an overview of methods for plant species, mainly species with high economic value, to achieve in vitro propagation through somatic embryogenesis. Chapters guide readers through somatic embryogenesis protocols, somatic embryos in plants, and new methodologies that are used out to solve in vitro recalcitrance of many species to this morphogenetic route. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Somatic Embryogenesis: Methods and Protocols aims to guide future researcher to achieve success in obtaining and regenerating somatic embryos.

Chapter 6 is available open access under a Creative Commons Attribution 4.0 International License vialink.springer.com.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Overview of Somatic Embryogenesis.- Internal and External Regulatory Elements Controlling Somatic Embryogenesis in Catharanthus: A Model Medicinal Plant.- Advances in Somatic Embryogenesis in Vanilla (Vanilla planifolia Jacks.).- Somatic Embryogenesis in Pines.- Use of thin cell layer technique for induction of somatic embryogenesis of Agave fourcroydes.- Theobroma Cacao: Somatic Embryogenesis.- Somatic Embryogenesis in Sugarcane ( Saccharum spp.).- Somatic embryogenesis in banana ( Musa spp.).- Citrus Protoplast Isolation and Plant Regeneration Through Somatic Embryogenesis.- Somatic embryogenesis in citrus ( Citrus spp.), var. Valencia.- Somatic Embryogenesis from Immature Olive zygotic embryos.- Somatic embryogenesis in papaya ( Carica papaya L.).- Somatic Embryogenesis of Anthurium andraeanum Linden. - A Tropical Florists' Plant.- Use of Thin Cell Layer (TCL) to obtain somatic embryogenesis.- Inorganic Compounds that Aid in Obtaining Somatic Embryos.- In vitro Stress-Mediated Somatic Embryogenesis in Plants.- Protocol of Histological Procedures During Coconut Somatic Embryogenesis.- Somatic Embryogenesis of Brachiaria brizantha (Syn. Urochloa brizantha ) analyzed by in situ hybridization.- Perspectives of Somatic Embryogenesis: Concluding Remarks.