SiRNA Delivery Methods

This volume provides a collection of cutting-edge strategies in siRNA delivery that were developed and refined over the years with tried-and-true methods. Written by a team of internationally renowned authors, this book describes, in detail, a variety of successful siRNA delivery methods, including peptide-based nanoparticles, liposomes, exosomes, polymers, aptamers, and viral vehicles. Written in the highly successful Methods in Molecular Biology series format, each proven protocol includes brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, SiRNA Delivery Methods: Methods and Protocols, will provide researchers, educators, clinicians, and biotechnology specialists with a broad understanding of the issues in siRNA delivery and how they can be overcome strategically.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Synthesis and Conjugation of Small Interfering RiboNucleic Neutral siRNNs.- Liver-Targeted siRNA Delivery Using Biodegradable Poly(amide) Polymer Conjugates.- PepFect6 Mediated siRNA Delivery into Organotypic Cultures.- Highly Efficient siRNA Delivery Mediated by Cationic Helical Polypeptides and Polypeptide-Based Nanosystems.- Disulfide-Bridged Cleavable PEGylation of Poly-L-Lysine for siRNA Delivery.- Preparation of a Cyclic RGD: Modified Liposomal siRNA Formulation for Use in Active Targeting to Tumor and Tumor Endothelial Cells.- A Multifunctional Envelope-Type Nano Device Containing A pH-Sensitive Cationic Lipid for Efficient Delivery of Short Interfering RNA to Hepatocytes In Vivo.- Bioreducible Poly(Beta-Amino Ester)s for Intracellular Delivery of siRNA.- Preparation of Polyion Complex Micelles Using Block Copolymers for siRNA Delivery.- Delivery of Small Interfering RNAs to Cells via Exosomes.- Dendrimer Nanovectors for siRNA Delivery.- Chitosan Nanoparticles for siRNA Delivery In Vitro.- Non-Covalently Functionalized of Single-Walled Carbon Nanotubes by DSPE-PEG-PEI for siRNA Delivery.- siRNA In Vivo Targeted Delivery to Murine Dendritic Cells by Oral Administration of Recombinant Yeast.- TLR9 Targeted siRNA Delivery In Vivo.- Aptamer-miRNA Conjugates for Cancer Cells Targeted Delivery.- Method for Confirming Cytoplasmic Delivery of RNA Aptamers.- Hapten-Binding Bispecific Antibodies for Targeted Delivery of siRNA and siRNA-Containing Nanoparticles.- Stable Delivery of CCR5 Directed shRNA into Human Primary Peripheral Blood Mononuclear Cells and Hematopoietic Stem/Progenitor Cells via a Lentiviral Vector.- Hepatic Delivery of Artificial Micro RNAs Using Helper-Dependent Adenoviral Vectors.- Intravascular AAV9 Administration for Delivering RNA Silencing Constructs to the CNS and Periphery.- Efficient Gene Suppression in Dorsal Root Ganglia (DRG) and Spinal Cord Using Adeno-Associated Virus (AAV) Vector EncodingShort-Hairpin RNA.- Synthetic siRNA Delivery: Progress and Prospects.