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SiRNA Delivery Methods

  • Kartonierter Einband
  • 313 Seiten
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This volume provides a collection of cutting-edge strategies in siRNA delivery that were developed and refined over the years with... Weiterlesen
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This volume provides a collection of cutting-edge strategies in siRNA delivery that were developed and refined over the years with tried-and-true methods. Written by a team of internationally renowned authors, this book describes, in detail, a variety of successful siRNA delivery methods, including peptide-based nanoparticles, liposomes, exosomes, polymers, aptamers, and viral vehicles. Written in the highly successful Methods in Molecular Biology series format, each proven protocol includes brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, SiRNA Delivery Methods: Methods and Protocols, will provide researchers, educators, clinicians, and biotechnology specialists with a broad understanding of the issues in siRNA delivery and how they can be overcome strategically.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts


1. Synthesis and Conjugation of Small Interfering RiboNucleic Neutral siRNNs
Alexander S. Hamil and Steven F. Dowdy

2. Liver-Targeted siRNA Delivery Using Biodegradable Poly(amide) Polymer Conjugates
Stephanie E. Barrett and Erin N. Guidry

3. PepFect6 Mediated siRNA Delivery into Organotypic Cultures
Suvarna Dash-Wagh, Ülo Langel, and Mats Ulfendahl

4. Highly Efficient siRNA Delivery Mediated by Cationic Helical Polypeptides and Polypeptide-Based Nanosystems
Lichen Yin, Nan Zheng, and Jianjun Cheng

5. Disulfide-Bridged Cleavable PEGylation of Poly-L-Lysine for siRNA Delivery
Min Tang, Haiqing Dong, Xiaojun Cai, Haiyan Zhu, Tianbin Ren, and Yongyong Li

6. Preparation of a Cyclic RGD: Modified Liposomal siRNA Formulation for Use in Active Targeting to Tumor and Tumor Endothelial Cells
Yu Sakurai, Tomoya Hada, and Hideyoshi Harashima

7. A Multifunctional Envelope-Type Nano Device Containing A pH-Sensitive Cationic Lipid for Efficient Delivery of Short Interfering RNA to Hepatocytes In Vivo
Yusuke Sato, Hideyoshi Harashima, and Michinori Kohara

8. Bioreducible Poly(Beta-Amino Ester)s for Intracellular Delivery of siRNA
Kristen L. Kozielski and

Jordan J. Green

9. Preparation of Polyion Complex Micelles Using Block Copolymers for siRNA Delivery
Hyun Jin Kim, Meng Zheng, Kanjiro Miyata, and Kazunori Kataoka

10. Delivery of Small Interfering RNAs to Cells via Exosomes
Jessica Wahlgren, Luisa Statello, Gabriel Skogberg, Esbjörn Telemo, and Hadi Valadi

11. Dendrimer Nanovectors for siRNA Delivery
Xiaoxuan Liu and Ling Peng

12. Chitosan Nanoparticles for siRNA Delivery In Vitro
Héloïse Ragelle, Kevin Vanvarenberg, Gaëlle Vandermeulen, and Véronique Préat

13. Non-Covalently Functionalized of Single-Walled Carbon Nanotubes by DSPE-PEG-PEI for siRNA Delivery
King Sun Siu
, James Koropatnick, and Wei-Ping Min

14. siRNA In Vivo Targeted Delivery to Murine Dendritic Cells by Oral Administration of Recombinant Yeast
Kun Xu, Zhongtian Liu, Long Zhang, Tingting Zhang, and Zhiying Zhang

15. TLR9 Targeted siRNA Delivery In

Dewan Md Sakib Hossain, Dayson Moreira, Qifang Zhang, Sergey Nechaev, Piotr Swiderski, and Marcin Kortylewski

16. Aptamer-miRNA Conjugates for Cancer Cells Targeted Delivery
Carla L. Esposito, Silvia Catuogno, and Vittorio de Franciscis

17. Method for Confirming Cytoplasmic Delivery of RNA Aptamers
David D. Dickey, Justin P. Dassie, and Paloma H. Giangrande

18. Hapten-Binding Bispecific Antibodies for Targeted Delivery of siRNA and siRNA-Containing Nanoparticles
Irmgard S. Thorey, Michael Grote, Klaus Mayer, and Ulrich Brinkmann

19. Stable Delivery of CCR5 Directed shRNA into Human Primary Peripheral Blood Mononuclear Cells and Hematopoietic Stem/Progenitor Cells via a Lentiviral Vector
Saki Shimizu, Swati Seth Yadav, and Dong Sung An

20. Hepatic Delivery of Artificial Micro RNAs Using Helper-Dependent Adenoviral Vectors
Carol Crowther, Betty Mowa, and Patrick Arbuthnot

21. Intravascular AAV9 Administration for Delivering RNA Silencing Constructs to the CNS and Periphery
Brett D. Dufour and Jodi L. McBride

22. Efficient Gene Suppression in Dorsal Root Ganglia (DRG) and Spinal Cord Using Adeno-Associated Virus (AAV) Vector Encoding Short-Hairpin RNA
Mitsuhiro Enomoto, Takashi Hirai, Hidetoshi Kaburagi, and

Takanori Yokota

23. Synthetic siRNA Delivery: Progress and Prospects
Thomas C. Roberts, Kariem Ezzat, Samir EL Andaloussi , and Marc S. Weinberg


Titel: SiRNA Delivery Methods
Untertitel: Methods and Protocols
EAN: 9781493949106
ISBN: 978-1-4939-4910-6
Format: Kartonierter Einband
Herausgeber: Springer, Berlin
Genre: Medizin
Anzahl Seiten: 313
Gewicht: 554g
Größe: H12mm x B258mm x T187mm
Jahr: 2016
Auflage: Softcover reprint of the original 1st ed. 2016

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