Serum/Plasma Proteomics

Blood science has become a cornerstone of multiple disciplines. This book, contributed to by leading experts in the field, provides a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics.

Blood science has become a cornerstone of multiple disciplines, including clinical chemistry, disease diagnosis, and therapeutic monitoring. Over the past decade, we have witnessed the advent of increasingly powerful proteomics technologies that allow greater fundamental insights into the blood proteome. These technological improvements have, in part, fuelled the quest for the discovery of novel blood-based biomarkers of disease. Serum/Plasma Proteomics: Methods and Protocols is a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics. Divided into five convenient sections, this detailed volume covers fractionation strategies for in-depth blood proteome analysis, defined procedures for blood collection, handling and storage, detailed protocols for performing both antibody-based and non-antibody based quantitative assays, proteome analysis of blood cell compartments, circulating nanomebraneous vesicles and blood-related fluids, and finally data management, statistical design, and bioinformatic challenges. This book, contributed to by leading experts in the field, provides a valuable foundation for the development and application of blood-based proteomics. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Serum/Plasma Proteomics : Methods and Protocols , with its well-honed methodologies, seeks to serve both professionals and investigators new to thefield in an effort to further our knowledge of this fundamental science.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from experts

Inhalt

Fractionation Strategies for In-depth Blood Proteome Analysis. Plasma Biomarker Discovery using 3-D Protein Profiling Coupled with Label-free Quantitation. Intact Protein Separation by 1 and 2-dimension Liquid Chromatography for Comparative Proteomic Separation of Partitioned Serum or Plasma. In-depth Analysis of a Plasma or Serum Proteome using a 4-D Protein Profiling Method. Intact-protein Analysis System (IPAS) for Discovery of Serum-based Disease Biomarkers. Model-based Discovery of Circulating Biomarkers. Low-molecular Weight Plasma Proteome Analysis using Centrifugal Ultrafiltration. High-throughput Analysis of Glycoproteins from Plasma.- Blood Collection and Handling Strategies. Minimizing Pre-analytical Variation of Plasma Samples by Proper Blood Collection and Handling. Collection and Handling of Blood Specimens for Peptidomics. Investigation of Peptide Biomarker Stability in Plasma Samples using Time-course MS Analysis.- Methods for Quantitative Assaying of Blood-Based Biomarkers. Biomarker Validation in Blood Specimens by Selected Reaction Monitoring Mass Spectrometry of N-glycosites. A Fluorescent Microsphere-based Method for Assay of Multiple Analytes in Plasma. Immuno-mass Spectrometry: Quantification of Low Abundance Proteins in Biological Fluids. Qualitative and Quantitative Analysis of Glycated Proteins in Human Plasma by Glucose Isotopic Labeling with 13 C 6 -reducing Sugars.- Proteome Analysis of Blood Cell Components, Vesicles, and Blood-related Fluids. Exosome Isolation for Proteomic Analyses and RNA Profiling. Extraction and Proteome Analysis of Liver Tissue Interstitial Fluid. A Protocol for the Preparation of Cryoprecipitate and Cryo-depleted Plasma.- Preparation of Platelet Concentrates. Phosphoproteome Analysis of the Platelet Plasma Membrane.- Bioinformatic Analysis of Blood Proteins and Peptides.Statistical Design and Analysis of Label-free LC-MS Proteomic Experiments: A Case Study of Coronary Artery Disease. Data Management in Mass Spectrometry-based Proteomics. Bioinformatics Challenges in the Proteomic Analysis of Human Plasma. Using the Human Plasma PeptideAtlas to Study Human Plasma Proteins.