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Serum/Plasma Proteomics

  • Kartonierter Einband
  • 412 Seiten
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Blood science has become a cornerstone of multiple disciplines. This book, contributed to by leading experts in the field, provide... Weiterlesen
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Blood science has become a cornerstone of multiple disciplines. This book, contributed to by leading experts in the field, provides a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics.

Blood science has become a cornerstone of multiple disciplines, including clinical chemistry, disease diagnosis, and therapeutic monitoring. Over the past decade, we have witnessed the advent of increasingly powerful proteomics technologies that allow greater fundamental insights into the blood proteome. These technological improvements have, in part, fuelled the quest for the discovery of novel blood-based biomarkers of disease. Serum/Plasma Proteomics: Methods and Protocols is a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics. Divided into five convenient sections, this detailed volume covers fractionation strategies for in-depth blood proteome analysis, defined procedures for blood collection, handling and storage, detailed protocols for performing both antibody-based and non-antibody based quantitative assays, proteome analysis of blood cell compartments, circulating nanomebraneous vesicles and blood-related fluids, and finally data management, statistical design, and bioinformatic challenges. This book, contributed to by leading experts in the field, provides a valuable foundation for the development and application of blood-based proteomics. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Serum/Plasma Proteomics : Methods and Protocols, with its well-honed methodologies, seeks to serve both professionals and investigators new to the field in an effort to further our knowledge of this fundamental science.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from experts

Part I. Fractionation Strategies for In-depth Blood Proteome Analysis 1. Plasma Biomarker Discovery using 3-D Protein Profiling Coupled with Label-free Quantitation Lynn A. Beer, Hsin-Yao Tang, Kurt T. Barnhart, and David W. Speicher 2. Intact Protein Separation by 1 and 2-dimension Liquid Chromatography for Comparative Proteomic Separation of Partitioned Serum or Plasma Simon Sheng, Helena Skalnikova, Andrew Meng, Qin Fu, Allen Everett, and Jennifer E. Van Eyk 3. In-depth Analysis of a Plasma or Serum Proteome using a 4-D Protein Profiling Method Hsin-Yao Tang, Huan Wang, Lynn A. Beer, and David W. Speicher 4. Intact-protein Analysis System (IPAS) for Discovery of Serum-based Disease Biomarkers Hong Wang and Samir Hanash 5. Model-based Discovery of Circulating Biomarkers Maryann S. Vogelsang and Parag Mallick 6. Low-molecular Weight Plasma Proteome Analysis using Centrifugal Ultrafiltration David W. Greening and Richard J. Simpson 7. High-throughput Analysis of Glycoproteins from Plasma Yan Li and Hui Zhang Part II. Blood Collection and Handling Strategies 8. Minimizing Pre-analytical Variation of Plasma Samples by Proper Blood Collection and Handling Jizu Yi, David Craft, and Craig A. Gelfand 9. Collection and Handling of Blood Specimens for Peptidomics Harald Tammen and Rüdiger Hess 10. Investigation of Peptide Biomarker Stability in Plasma Samples using Time-course MS Analysis Jizu Yi, Zhaoxia Liu, Craig A. Gelfand, and David Craft Part III. Methods for Quantitative Assaying of Blood-Based Biomarkers 11. Biomarker Validation in Blood Specimens by Selected Reaction Monitoring Mass Spectrometry of N-glycosites Reto Ossola, Ralph Schiess, Paola Picotti, Oliver Rinner, Lukas Reiter, and Ruedi Aebersold 12. A Fluorescent Microsphere-based Method for Assay of Multiple Analytes in Plasma Oliver K. Bernhard, Rommel A. Mathias, Thomas W. Barnes, and Richard J. Simpson 13. Immuno-mass Spectrometry: Quantification of Low Abundance Proteins in Biological Fluids Vathany Kulasingam, Christopher R. Smith, Ihor Batruch, and Eleftherios P. Diamandis 14. Qualitative and Quantitative Analysis of Glycated Proteins in Human Plasma by Glucose Isotopic Labeling with 13C6-reducing Sugars Feliciano Priego-Capote, Maria Ramírez-Boo, Denis Hochstrasser, and Jean-Charles Sanchez Part IV. Proteome Analysis of Blood Cell Components, Vesicles, and Blood-related Fluids 15. Exosome Isolation for Proteomic Analyses and RNA Profiling Douglas D. Taylor, Wolfgang Zacharias, and Cicek Gercel-Taylor 16. Extraction and Proteome Analysis of Liver Tissue Interstitial Fluid Wei Sun, Ying Jiang, and Fuchu He 17. A Protocol for the Preparation of Cryoprecipitate and Cryo-depleted Plasma Rosemary L. Sparrow, David W. Greening, and Richard J. Simpson 18. Preparation of Platelet Concentrates David W. Greening, Richard J. Simpson, and Rosemary L. Sparrow 19. Phosphoproteome Analysis of the Platelet Plasma Membrane Thomas Premsler, Urs Lewandrowski, Albert Sickmann, and René Peiman Zahedi Part V. Bioinformatic Analysis of Blood Proteins and Peptides 20. Statistical Design and Analysis of Label-free LC-MS Proteomic Experiments: A Case Study of Coronary Artery Disease Timothy Clough, Siegmund Braun, Vladimir Fokin, Ilka Ott, Susanne Ragg, Gunther Schadow, and Olga Vitek 21. Data Management in Mass Spectrometry-based Proteomics Lennart Martens 22. Bioinformatics Challenges in the Proteomic Analysis of Human Plasma Joseph M. Foster and Lennart Martens 23. Using the Human Plasma PeptideAtlas to Study Human Plasma Proteins Terry Farrah, Eric W. Deutsch, and Ruedi Aebersold Appendix I Standard operating procedures for plasma collection in clinical research Tuck et al., (2009). J Proteome Res 8, 113-7 Appendix II Standard operating procedures for serum collection in clinical research Tuck et al., (2009). J Proteome Res 8, 113-7 Appendix III Measured concentrations of plasma proteins from quantitative assays Haab et al., (2005). Proteomics 5, 3278-91 Appendix IV Plasma proteome reference source Appendix V Reference ranges for blood testsHäggström (2010). Uppsala University Hospital ("Laborationslista"). Artnr 40284 Sj74a. Issued on April 22, 2008.


Titel: Serum/Plasma Proteomics
Untertitel: Methods and Protocols
EAN: 9781493958016
ISBN: 1493958011
Format: Kartonierter Einband
Herausgeber: Humana Press
Anzahl Seiten: 412
Gewicht: 771g
Größe: H254mm x B178mm x T22mm
Jahr: 2016
Auflage: Softcover reprint of the original 1st ed. 2011

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