Reflecting a vast amount of new information concerning the functional characteristics of the various 5-HT receptor subtypes and the Na + -dependent serotonin transporter (SERT), this volume provides state of the art methodologies currently applied in serotonin research from leading experts in the field. Serotonin Receptor Technologies describes approaches that vary from molecular biological and biochemical techniques (e.g., regarding receptor dimerization), fluorescence microscopy and imaging applications, flow cytometry, the use of organotypic slice and cell cultures to the generation of genetically modified animal models and the development of sophisticated behavioral tests, thus covering a wide spectrum of techniques to study serotonergic signaling in detail. Written for the popular Neuromethods series, chapters include the kind of detail and practical advice that ensures successful results in the lab.

Authoritative and convenient, Serotonin Receptor Technologies serves to foster both basic and translational research aiming to further deepen our understanding of the various facets of aminergic systems, as well as to aid research on similar problems with related GPCRs and neurotransmitter transporters.

Includes cutting-edge methods and protocols involving the serotonergic system Provides vital details essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Novel Approaches to Serotonin Receptor Interaction Studies.- Techniques for the Study of GPCR Heteromerization in Living Cells and Animal Models.- Organotypic Slices and Biolistic Transfection for the Study of Serotonin Receptor Function in CNS Neurons.- Dissecting a Model of Depressive-Related Phenotype and Antidepressants Effects in 129S2/SvPas Mice.- The Murine Serotonin Syndrome and the 5-HT 1A Receptor: Behavioral Effects and Hypothermia.- 5-HT 4 Receptor Subtype, -arrestin Level, and Rapid Onset Effects of Antidepressant Drugs.- Autoshaping Memory Formation and Retention Loss: Are Serotonin and Other Neurotransmitter Transporters Involved?.- Flow Cytometry to Determine Serotonin Transporter Function in Human Peripheral Blood Cells.- Functional Genomics of Serotonin Receptors in Helminth Parasites: Elucidation of Receptor Function through RNA Interference (RNAi).- Visualization of the Serotonin System in Drosophila Brain: Immunofluorescence and Confocal Microscopy.- Serotonin Modulates Adult Neurogenesis in an Invertebrate Model: Approaches to Receptor Localization and Function.- Immunolocalization of Serotonergic Neurons in Arthropod Developmental and Phylogenetic Neuroanatomy.