High-performance liquid chromatography (HPLC) is a widely used separation method. Its efficacy and accuracy depends on a number of factors, including the often overlooked experimental parameter of solvent selection. This practical book offers an overview of the role of the solvent in HPLC as well as detailed discussions of seven different classes of solvent.

Informationen zum Autor Paul C. Sadek is President of Analytical Consulting Laboratories, a manufacturing and research facility located in Kentwood, Michigan. Previously, he spent more than fifteen years in academia and industry, including many years in research and development with Burdick Jackson, the world's premier solvent manufacturer. Dr. Sadek also wrote Troubleshooting HPLC Systems: A Bench Manual. Klappentext High-performance liquid chromatography (HPLC) is a procedure for separating components from a mixture of chemical substances; a combination of separation, identification, and quantitative measurements. Solvent selection is perhaps the most commonly overlooked parameter in HPLC. Even the most experienced analytical chemist tends to select one of three familiar solvents. The HPLC Solvent Guide provides detailed coverage of all commonly used HPLC solvents used in a wide range of separations. HPLC is a mature but substantial market, and one that Wiley reaches successfully and well. The HPLC list is established, and this second edition of a successful title will build upon the success of the first. This is a revised and expanded edition in a field that is still growing into areas of analysis and methods. Zusammenfassung High-performance liquid chromatography (HPLC) is a procedure for separating components from a mixture of chemical substances. Solvent selection is the most commonly overlooked parameter of HPLC. This book reviews the role of solvents in this procedure, providing an easy reference for solvent class, field of application, and specific analyte class. Inhaltsverzeichnis Preface. Introduction. Abbreviations, Acronyms, and Symbols. Chapter 1: Physical and Chemical Solvent Properties. Chapter 2: Method Optimization. Chapter 3: Method Validation and Ongoing Performance Evaluation. Chapter 4: Alcohols. Chapter 5: Alkanes and Alkyl Aromatics. Chapter 6: Chlorinated Alkanes and Chlorinated Benzenes. Chapter 7: Ethers. Chapter 8: Keotones and Esters. Chapter 9: Nitriles and Nitrogenous Solvents. Chapter 10: Water, Dimethyl Sulfoxide, and Common Acidic Modifiers. References. Index....

Klappentext

High-performance liquid chromatography (HPLC) is a procedure for separating components from a mixture of chemical substances; a combination of separation, identification, and quantitative measurements. Solvent selection is perhaps the most commonly overlooked parameter in HPLC. Even the most experienced analytical chemist tends to select one of three familiar solvents. The HPLC Solvent Guide provides detailed coverage of all commonly used HPLC solvents used in a wide range of separations.

HPLC is a mature but substantial market, and one that Wiley reaches successfully and well. The HPLC list is established, and this second edition of a successful title will build upon the success of the first. This is a revised and expanded edition in a field that is still growing into areas of analysis and methods.

Inhalt

Preface.

Introduction.

Abbreviations, Acronyms, and Symbols.

Chapter 1: Physical and Chemical Solvent Properties.

Chapter 2: Method Optimization.

Chapter 3: Method Validation and Ongoing Performance Evaluation.

Chapter 4: Alcohols.

Chapter 5: Alkanes and Alkyl Aromatics.

Chapter 6: Chlorinated Alkanes and Chlorinated Benzenes.

Chapter 7: Ethers.

Chapter 8: Keotones and Esters.

Chapter 9: Nitriles and Nitrogenous Solvents.

Chapter 10: Water, Dimethyl Sulfoxide, and Common Acidic Modifiers.

References.

Index.