RNAi

RNA interference (RNAi), in which RNA silences RNA, is the most recent discovery to revolutionize the study of biology. In RNAi: Design and Applications, leaders in the field contribute state-of-the-art, easy to follow methods and bench protocols designed for practical, everyday use of RNAi in biological research. Divided into two parts, this comprehensive volume covers fundamentals including designs of RNAi, biochemical assay protocols for the major components of RNAi, and study of potential off-target effects, followed by an extensive section covering various applications of RNAi in diverse model organisms and systems, from antiviral and anticancer applications to altering flower color in plants.

Cutting edge and clearly written, RNAi: Design and Applications enables a researcher with standard molecular biological training to perform major RNAi-related experiments and contribute to this revolutionary, growing field.

Step-by-step instructions in a laboratory bench protocol format Detailed lists of the necessary equipment and reagents Tips on troubleshooting from actual experience of the experts Example data set in most chapters Variety of organisms and applications

Inhalt
Application of RNAi in Diverse Organisms.- Principles of Dicer Substrate (D-siRNA) Design and Function.- Expression, Purification, and Analysis of Recombinant Drosophila Dicer-1 and Dicer-2 Enzymes.- In vitro RNA Cleavage Assay for Argonaute-Family Proteins.- Identifying siRNA-Induced Off-Targets by Microarray Analysis.- Application of RNAi in Diverse Organisms.- Hydrodynamic Delivery of siRNA in a Mouse Model of Sepsis.- Nasal Delivery of siRNA.- RNA Interference as a Genetic Tool in Trypanosomes.- Lentivirus-Mediated RNA Interference in Mammalian Neurons.- Silencing Genes by RNA Interference in the Protozoan Parasite Entamoeba histolytica.- Use of RNAi in C. elegans.- Application of siRNA Against SARS in the Rhesus Macaque Model.- siRNA and shRNA as Anticancer Agents in a Cervical Cancer Model.- Identification and Expression Analysis of Small RNAs During Development.- Screening and Identification of Virus-Encoded RNA Silencing Suppressors.- Application of RNA Interference in Functional Genomics Studies of a Social Insect.- Temporal Control of Gene Silencing by in ovo Electroporation.- Altering Flower Color in Transgenic Plants by RNAi-Mediated Engineering of Flavonoid Biosynthetic Pathway.- Transgenic RNA Interference in Mice.