This volume provides readers with the latest information on the advances made in the field of rhodopsins. The chapters in this book cover topics such as new discoveries and developments; new ways to search for rhodopsins; methods to characterize the function and structure of rhodopsins on a molecular level; nano volume high throughput in meso crystallization, and fourth generation x-ray synchrotron sources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Rhodopsin: Methods and Protocols is a valuable resource for any scientist and researcher interested in learning more about this developing field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Microbial Rhodopsins.- Molecular Biology of Microbial Rhodopsins.- Rhodopsin-Based Optogenetics: Basics and Applications.- Searching Metagenomes for New Rhodopsins.- E. coli Expression and Purification of Microbial and Viral Rhodopsins.- Crystallization of Microbial Rhodopsins.- Crystallographic Studies of Rhodopsins: Structure and Dynamics.- Time-Resolved UV-VIS Spectroscopy of Microbial Rhodopsins.- Solid-State NMR Spectroscopy on Microbial Rhodopsins.- FTIR and Raman Spectroscopy of Rhodopsins.- Single Cell Resolution Optogenetics via Expression of Soma-Targeted Rhodopsins.- Electrophysiological Characterization of Microbial Rhodopsins Transport Properties: Electrometric and pH Measurements using Planar Lipid Bilayer, Collodion Film and Fluorescent Probes Approaches.- Electrophysiological Characterization of Microbial Rhodopsins by Patch-Clamp Experiments.- Molecular Optimization of Rhodopsin-Based Tools for Neuroscience Applications.- Optogenetic Studies of Mitochondria.- In Vivo and In Vitro Characterization of Cyclase and Phosphodiesterase Rhodopsins.- Optogenetic Control of Human Stem Cell-Derived Neurons.