This book provides practical guidance on all aspects of reverse phase protein array (RPPA) technology, which permits the quantification of protein levels in cell or tissue lysates. In addition, the latest results are presented from laboratories across the world where experts are successfully running the challenging RPPA data platform. After an introductory chapter by the inventor of the RPPA technique, the demanding task of lysate preparation is explained. Subsequent chapters review printing platforms and analytical platforms and discuss antibody screening in detail. The role of RPPA data in integrative analyses is thoroughly examined, and the various applications of RPPA, for example to identify molecular targeting drugs or evaluate cancer drug efficacy, are discussed with reference to the latest knowledge. It is hoped that the book will foster the implementation of RPPA by documenting the practical details of the technology and by revealing its great potential. It will be of high value for researchers who plan to build an RPPA platform in their institute and for researchers, clinicians, and companies who use RPPA through collaboration with an existing RPPA facility.

Guidance on handling of samples for stable data generation Explanation of the role of RPPA data in integrative analyses Expert opinions and updates on recent results from RPPA

Autorentext
Tesshi Yamada, M.D., Ph.D.

Professor of International University of Health and Welfare, Narita, Japan

Satoshi Nishizuka, M.D., Ph.D.

Professor of Iwate Medical University, Japan

Gordon Mills, M.D., Ph.D.

Professor of University of Texas, MD Anderson Cancer Center

Lance Liotta, M.D., Ph.D.

Co-Director of the Center for Applied Proteomics and Molecular Medicine, George Mason University

Inhalt
Chapter 1: Origin and future of RPPA technology.- Chapter 2: Lysate preparation.- Chapter 3: Printing platforms 1.- Chapter 4: Printing platforms 2.- Chapter 5: Analytical platforms 1.- Chapter 6: Analytical platforms 2.- Chapter 7: Analytical platforms 3.- Chapter 8. Antibody screening 1.- Chapter 9. Antibody screening 2.- Chapter 10. Antibody screening 3.- Chapter 11. Integrative analysis for genomics and RPPA.- Chapter 12. Integrative analysis of multiple platforms of RPPA.- Chapter 13. Drug screening platforms and RPPA.- Chapter 14. RPPA in small population analysis.- Chapter 15: Molecular target identification using RPPA.- Chapter 16: RPPA in clinical trials.- Chapter 17: RPPA and future medicine.