Gold(I,III) Complexes Designed for Selective Targeting and Inhibition of Zinc Finger Proteins

This book examines Au (I, III) complexes that selectively attack and inhibit zinc finger proteins (ZnFs) for potential therapeutic use. The author explores gold(I)-phosphine, gold(III) complexes with N^N and C^N donors as inhibitors of the HIV-1 nucleocapsid protein (NCp7), in comparison to the human transcription factor Sp1. To determine the coordination sphere of the gold adducts formed by interaction with ZnFs, two innovative approaches are used, based on Travelling-Wave Ion Mobility coupled with Mass Spectrometry (TWIM-MS), and X-ray Absorption Spectroscopy. Both approaches are proven to yield valuable structural information regarding the coordination sphere of gold in the adducts. In addition, the organometallic compound [Au (bnpy)Cl2] is evaluated. The system is shown to be capable of inhibiting ZnFs by means of CS coupling.

Nominated as an outstanding Ph.D. thesis by the University of Campinas, Brazil Presents an innovative approach, using gold chemistry to target zinc finger proteins Explores the use of Travelling-Wave Ion Mobility coupled with Mass Spectrometry, and X-ray Absorption Spectroscopy to obtain structural information on the Gold adducts

Inhalt

Au(I)-phosphine series design.- Probing the Protein: Ion Mobility Spectrometry.- Probing Gold: X-ray Absorption Spectroscopy.- Probing Cells: Cytotoxicity Evaluation.- Au(III) series with k 2 C,N and k 2 N,N' ligands.- "Dual Probe" X-ray Absorption Spectroscopy.