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Quantitative Proteomics by Mass Spectrometry

  • Fester Einband
  • 306 Seiten
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This volume describes prominent methodologies developed by laboratories that have been leading the field of quantitative proteomic... Weiterlesen
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Beschreibung

This volume describes prominent methodologies developed by laboratories that have been leading the field of quantitative proteomics by mass spectrometry. The procedures for performing the experiments are described in an easy-to-understand manner with many technical details that usually are not reported in typical research articles. This second edition of Quantitative Proteomics by Mass Spectrometry provides a broad perspective of the methodologies used for quantifying proteins and post-translational modifications in different types of biomedical specimens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Quantitative Proteomics by Mass Spectrometry, Second Edition is a valuable resource to help researchers understand and learn about the latest tools used in the study of quantitative proteomics by mass spectrometry.




Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts



Inhalt
1. Increased Depth and Breadth of Plasma Protein Quantitation via Two Dimensional Liquid Chromatography/Multiple Reaction Monitoring-Mass Spectrometry with Labeled Peptide StandardsAndrew J. Percy, Juncong Yang, Andrew G. Chambers, and Christoph H. Borchers2. Quantitative Analysis of the Wirt5-Regulated Lysine Succinylation Proteome in Mammalian CellsYue Chen3. Determining the Composition and Stability of Protein Complexes Using an Integrated Label-Free and Stable Isotope Labeling StrategyTodd M. Greco, Amanda J. Guise, and Ileana M. Cristea4. Label-Free Quantitation for Clinical ProteomicsRobert Moulder, Young Ah Goo, and David R. Goodlett5. Proteogenomic Methods to Improve Genome AnnotationKeshava K. Datta, Anil K. Madugundu, and Harsha Gowda6. Mass Spectrometry-Based Quantitative O-GlcNAcomic AnalysisJunfeng Ma and Gerald W. Hart7. Isolating and Quantifying Plasma HDL Proteins by Sequential Density Gradient Ultracentrifugation and Targeted ProteomicsClark M. Henderson, Tomas Vaisar, and Andrew N. Hoofnagle8. A Method for Label-Free Differential Top-Down ProteomicsIoanna Ntai, Timothy K. Toby, Richard D. LeDuc, and Neil L. Kelleher9. Multiplexed Immunoaffinity Enrichment of Peptides with Anti-Peptide Antibodies and Quantification by Stable Isotope Dilution Multiple Reaction Monitoring Mass SpectrometryEric Kuhn and Steven A. Carr10. High Throughput Quantitative Proteomics Enabled by Mass Defect-Based 12-Plex DiLeu Isobaric TagsDustin C. Frost and Lingjun Li11. Isotopic N, N-Dimethyl Leucine (iDiLeu) for Absolute Quantification of Peptides Using a Standard Curve ApproachTyler Greer and Lingjun Li12. Selecting Optimal Peptides for Targeted Proteomic Experiments in Human Plasma Using In Vitro Synthesized Proteins as Analytical StandardsJames G. Bollinger, Andrew B. Stergachis, Richard S. Johnson, Jarrett D. Egertson, and Michael J. MacCoss13. Using the CPTAC Assay Portal to Identify and Implement Highly Characterized Targeted Proteomics AssaysJeffrey R Whiteaker, Goran N. Halusa, Andrew N. Hoofnagle, Vagisha Sharma, Brendan MacLean, Ping Yan, John A. Wrobel, Jacob Kennedy, DR Mani, Lisa J. Zimmerman, Matthew R. Meyer, Mehdi Mesri, Susan E. Abbatiello, Emily Boja, Stven A. Carr, Daniel W. Chan, Xian Chen, Jing Chen, Sherri R. Davies, Matthew J. C. Ellis, David Fenyö, Tara Hiltke, Karen A. Ketchum, Chris Kinsinger, Eric Kuhn, Daniel C. Liebler, De Lin, Tao Liu, Michael Loss, Michael J. MacCoss, Wei-Jun Qian, Robert Rivers, Karin D. Rodland, Kelly V. Ruggles, Mitchell G. Scott, Richard D. Smith, Stefani Thomas, R. Reid Townsend, Gordon Whiteley, Chaochao Wu, Hui Zhang, Zhen Zhang, Henry Rodriguez, and Amanda G. Paulovich14. Large-Scale and Deep Quantitative Proteome Profiling Using Isobaric Lableing Coupled with Two-Dimensional LC-MS/MSMarina A. Gritsenko, Zhe Xu, Tao Liu, and Richard D. Smith15. Multiple and Selective Reaction Monitoring Using Triple Quadrupole Mass Spectrometer: Pre-Clinical Large Cohort AnalysisQin Fu, Zhaohui Chen, Shenyan Zhang, Sarah Parker, Zongming Fu, Xiaoqian Liu, Josef Coresh, and Jennifer E. Van Eyk16. Methods for SWATH™: Data Independent Acquisition on TripleTOF Mass SpectrometersRonald J. Holewinski, Sarah J. Parker, Andrea D. Matlock, Vidya Venkatraman, and Jennifer E. Van Eyk17. Measurement of Phosphorylated Peptides with Absolute QuantificationRaven J. Reddy, Timothy G. Curran, Yi Zhang, and Forest M. White18. Proteomic Analysis of Protein Turnover by Metabolic Whole Rodent Pulse-Chase Isotopic Labeling and Shotgun Mass Spectrometry AnalysisJeffrey N. Savas, Sung Kyu Park, and John R. Yates III

Produktinformationen

Titel: Quantitative Proteomics by Mass Spectrometry
Editor:
EAN: 9781493935222
ISBN: 978-1-4939-3522-2
Format: Fester Einband
Herausgeber: Springer, Berlin
Genre: Chemie
Anzahl Seiten: 306
Gewicht: g
Größe: H25mm x B261mm x T184mm
Jahr: 2016
Auflage: 2. Aufl.

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