Autorentext

Dr. Adalberto Pessoa Jr., Full professor at the School of Pharmaceutical Sciences, Biochemical-Pharmaceutical Technology Department, of the University of São Paulo and Visiting Professor at King's College London, Institute of Pharmaceutical Science. He has experience in fermentation technology and in the purification processes of biotechnological products of interest to the pharmaceutical and food industries.

Dr. Beatriz Vahan Kilikian, nowadays retired, she was Associate Professor at the Polytechnic School of the University of São Paulo, from 1983 to 2012. She has experience in microrganisms cultivation and in the purification processes of biotechnological products of interest to the pharmaceutical, chemical and food industries.

Dr. Paul Frederick Long Full Professor of Biotechnology at King's College London and Visiting International Research Professor at the University of São Paulo. Prof Long is a microbiologist by training and his research uses a combination of bioinformatics, laboratory and field studies to discover new medicines from nature, particularly from the marine environment.

Klappentext

This unique text provides a background and concepts related to the purification of biomolecules and with an industrial perspective.

Inhalt

Chapter 1 Introduction

Chapter 2 Purification processes: analytical methods and enzyme stability

Chapter 3 Cell Disruption

Chapter 4 Filtration and Centrifugation

Chapter 5 Membrane Separation Processes

Chapter 6 Protein Precipitation

Chapter 7 Liquid-liquid extraction in aqueous two-phase systems

Chapter 8 Introduction to chromatography

Chapter 9 Size exclusion chromatography

Chapter 10 Iion Exchange chromatography

Chapter 11 Hydrophobic interaction chromatography

Chapter 12 Affinity Chromatography

Chapter 13 Adsorption chromatography in monolithic columns

Chapter 14 Chromatography: expanded bed adsorption (EBA)

Chapter 15 Continuous Chromatography using a Simulated Moving Bed System

Chapter 16 Chromatography scale-up

Chapter 17 Crystallization

Chapter 18 Distillation

Chapter 19 Plasmid Purification

Chapter 20 Purification of monoclonal antibodies

Chapter 21 Peptides of biotechnological interest: general concepts, production, purification and chemical characterization

Chapter 22 Integration of steps in the production of biotechnological products