This fully-updated third edition provides systems-based methodologies and innovative technologies that can be used for solving complicated problems of complex systems. A wide range of theoretical and experimental approaches are introduced for practical applications, as the content explores the basic and novel concepts in psychoneuroimmunology (PNI), especially the relationships among stress, inflammation, and psychophysiological disorders, as well as various cutting-edge technologies and methods for PNI studies, including the utilizations of mouse models and vaccine models. As part of the Methods in Molecular Biology series, the chapters seek to provide a practical, state-of-the-art, and holistic view for the translation of PNI into better preventive and personalized medical practice.

Authoritative and hands-on, Psychoneuroimmunology: Methods and Protocols, Third Edition will be of use to biomedical students and professionals at all levels.

Contains key implementation advice from the experts Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results

Inhalt

Purpose in Life and Stress Reactivity.- Personality and Psychoneuroimmunology: A Systems Biology Perspective.- Personality, Aging, and Alzheimer's Disease: Implications for Psychoneuroimmunology and Personalized Medicine.- Psychoneuroimmunology of Mood Disorders.- Biomarkers Unveiling the Interplay of Mind, Nervous System, and Immunity.- Mitochondrial Functioning: Front and Center in Defining Psychosomatic Mechanisms of Allostasis in Health and Disease.- Psycho-Neuro-Endocrine-Immunology Therapy of Cancer, Autoimmunity, Geriatric Disorders, COVID-19, and Hypertension.- Hair and Saliva Endocannabinoid and Steroid Hormone Analysis by Liquid Chromatography Paired with Tandem Mass Spectrometry.- Measuring Vaccine Responses in The Multiplex Era.- Mouse Testing Methods in Psychoneuroimmunology: Measuring Behavioral Responses.- Methods for Modeling Early Life Stress in Rodents.- The MRL Model: A Valuable Tool in Studies of Autoimmunity-Brain Interactions.- A Computational Pipeline for Identifying Gene Regulatory Networks: A Case Study of Response to Exercise.- A Computational Protocol for the Knowledge-Based Assessment and Capture of Pathologies.- Microglial and Macrophage Plasticity and Regional Cerebral Blood Flow in the Prenatal Brain and Gut Under Vagus Nerve Stimulation.- Perinatal Psychoneuroimmunology of Prenatal Stress and Its Effects on Fetal and Postnatal Brain Development.- The Integrative Single-Case Design as A Biosemiotic-Systemic Research Tool in Psychoneuroimmunology.