Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants

Here, experts provide detailed protocols for establishing in vitro cultures of key medicinal plants and their mass multiplication in a controlled environment, as well as numerous other methodologies including large-scale bioreactor metabolite production.

Given the vital and far-reaching applications of medicinal plant metabolites worldwide, the quality and consistency of the products as well as the very survival of various species are of the utmost importance. In Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants , expert researchers provide detailed, step-by-step protocols for the establishment of in vitro cultures of key medicinal plants, their mass multiplication in a controlled environment, and step-wise secondary metabolite analysis, genetic transformation, large-scale metabolite production in a bioreactor, and molecular markers. In addition, many of these protocols will provide a basis for much needed efforts of in vitro germplasm conservation or cryopreservation of medicinal plant species at the brink of extinction as well as efforts to protect them from the adverse impact of rapid climatic changes. As a volume in the Methods in Molecular Biology ™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants is an ideal resource for scientists endeavoring to continue the research on this exciting natural branch of medicine.

Provides step-by-step protocols for the establishment of in vitro cultures of key medicinal plants and their mass multiplication in a controlled environment Provide step-wise secondary metabolite analysis, genetic transformation, large-scale metabolite production in a bioreactor, and molecular markers Includes supplementary material: sn.pub/extras

Inhalt
Micropropagation and Chemical Analysis.- Establishment of Adventitious Root Cultures of Echinacea purpurea for the Production of Caffeic Acid Derivatives.- In Vitro Propagation of Rauwolfia serpentina Using Liquid Medium, Assessment of Genetic Fidelity of Micropropagated Plants, and Simultaneous Quantitation of Reserpine, Ajmaline, and Ajmalicine.- Medicinal Properties, In Vitro Protocols and Secondary Metabolite Analyses of Scots Pine.- Saussurea medusa Cell Suspension Cultures for Flavonoid Production.- Large-scale In Vitro Multiplication of Crataeva nurvala.- Bilberry In Vitro Protocols and Analyses of Phenolic Compounds.- In Vitro Propagation of Two Tuberous Medicinal Plants: Holostemma ada-kodien and Ipomoea mauritiana.- In Vitro Production of Gymnemic Acid from Gymnema sylvestre (Retz) R. Br. Ex Roemer and Schultes Through Callus Culture Under Abiotic Stress Conditions.- Establishment of Plant Regeneration and Cryopreservation System from Zygotic Embryo-Derived Embryogenic Cell Suspension Cultures of Ranunculus kazusensis.- In Vitro Culture and Secondary Metabolite Isolation in Bryophytes.- Micropropagation and In Vitro Conservation of Vanilla (Vanilla planifolia Andrews).- Protocol for In Vitro Regeneration and Marker Glycoside Assessment in Swertia chirata Buch-Ham.- Protocols for Establishment of an In Vitro Collection of Medicinal Plants in the Genus Scutellaria.- Protocols for In Vitro Culture and Phytochemical Analysis of Phyllanthus Species (Euphorbiaceae).- In Vitro Clonal Propagation of Asparagus racemosus, a High Value Medicinal Plant.- Micropropagation of Penthorum chinenseThrough Axillary Buds.- Transgenic Approaches.- Spontaneous Plant Regeneration and Production of Secondary Metabolites from Hairy Root Cultures of Centaurium erythraea Rafn.- Transgenic Hypericum perforatum.- Agrobacterium-Mediated Transformation of Ruta graveolens L.- Gene Expression Profiling in Taxus baccata L. Seedlings and Cell Cultures.- Catapol Production in Chinese Foxglove (Rehmannia glutinosa Libos.) Hairy Roots Transformed with Agrobacterium rhizogenes ATCC15834.- Molecular Markers and Microsatellites.- Identification of Medicinal Plants and Plant Sequences: Multiplexed MLPA Assay.- Isolation of Microsatellites from Catharanthus roseus (L.) G. Don Using Enriched Libraries.- Biotransformation, Bioreactors, and Metabolomics.- Production of Cinnamyl Glycosides in Compact Callus Aggregate Cultures of Rhodiola rosea Through Biotransformation of Cinnamyl Alcohol.- Spearmint Plantlet Culture System as a Means to Study Secondary Metabolism.- Bioreactor Production of Secondary Metabolites from Cell Cultures of Periwinkle and Sandalwood.- Camptothecin Production by In Vitro Cultures and Plant Regeneration in Ophiorrhiza Species.- Metabolomic Analysis of Ocotea odorifera Cell Cultures: A Model Protocol for Acquiring Metabolite Data.- The Production of 9-methoxycanthin-6-one from Callus Cultures of (Eurycoma longifolia Jack) Tongkat Ali.- Altered Gravity and Biotechnology.- Plant Secondary Metabolism in Altered Gravity.- The Role of Biotechnology in the Production of the Anticancer Compound Podophyllotox.