Proteins are involved in all essential processes in a living organism or cell. For the understanding of the biological mechanisms at the molecular level it is necessary to study the protein stucture. This manual includes the most suitable, well established protocols for protein structure analysis that are in use worldwide in modern biochemical, biomedical or pharmaceutical research laboratories.

Klappentext

The structure of a protein is essential for its function. Therefore, a variety of methods for analyzing protein structure have been developed recently; the most advanced and sensitive techniques have been selected for this manual. The presented procedures are well established and have been found useful in many laboratories; they are easy to perform and thus also suitable for beginners.BREnclosed are protocols on the separation of proteins and peptides, manual and automated microsequencing, electrophoresis and blotting, analysis of amino acids, identification of cysteine residues and lipids as well as on mass spectrometry crystallization of macromolecules.

Inhalt
I Separation of Proteins.- 1 Purification of Proteins for Sequencing.- 2 Affinity Chromatography of Proteins.- 3 Protein Purification by Aqueous Two-Phase Systems.- 4 Rapid HPLC Separation of Proteins, Peptides, and Amino Acids Using Short Columns Filled with Nonporous Silica-Based Particles.- II Separation of Peptides.- 5 Enzymatic and Chemical Cleavages of Proteins.- 6 Separation of Peptides Using HPLC and TLC Fingerprints.- 7 Microseparation of In Situ Digested Peptides.- 8 Isolation of Peptides for Microsequencing by In-Gel Proteolytic Digestion.- III Manual and Automated Microsequencing Methods.- 9 Automated Microsequencing: Introduction and Overview.- 10 A Manual Method for Protein Sequence Analysis Using the DABITC/PITC Method.- 11 Solid-Phase Sequencing of Peptides and Proteins.- 12 Sequence Analysis of the NH2-Terminally Blocked Proteins Immobilized on PVDF Membranes from Polyacrylamide Gels.- IV Electrophoresis and Blotting of Proteins.- 13 Two-Dimensional Electrophoresis.- 14 Semi-Dry Blotting onto Hydrophobic Membranes.- V Analysis of Amino Acids.- 15 Highly Sensitive Amino Acid Analysis.- 16 Quantitative Analysis of D- and L-Amino Acids by HPLC.- VI Identification of Cysteine Residues and Lipids in Proteins.- 17 Identification of Cysteine Residues and Disulfide Bonds in Proteins.- 18 Lipid Modifications of Proteins.- VII Mass Spectrometry in Peptide and Protein Analysis.- 19 Mass Spectrometry in Peptide and Protein Sequence Analysis.- 20 Carboxy-Terminal Sequencing by Combining Carboxypeptidase Y and P Digestion with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry.- 21 Crystallization of Biological Macromolecules.