This book is devoted to recent advances in analysis of the molecular basis and dynamics of post-translational modifications (PTMs) of proteins for a comprehensive understanding of their key roles in cell signaling networks and diverse biological processes, and their perturbation in a variety of life-threatening diseases such as cancer and inflammatory diseases. The book includes research regarding PTMs and methods of their investigation derived from interdisciplinary collaborations between leading scientists in the fields of molecular, medical, proteomic, structural, and mathematical biology. This book consists of four sections. The first part focuses on recent advances in procedures for analysis of PTMs and cell signaling. The second part is devoted to mathematical simulation of signal transduction pathways and of the formation of protein complexes in living cells. The third part deals with structural and functional analyses of proteins involved in the regulation of PTMs and cell signaling. The fourth part describes cutting-edge findings regarding critical signal transduction pathways and their dysregulation in human diseases. This book is aimed at both established scientists and students in various fields of biology including molecular, cellular, structural, proteomic, and mathematical biology. Readers can access cutting-edge research and methodologies in their own field as well as interdisciplinary research that impacts on their field. The book can function as a reference for pharmaceutical scientists, biomedical researchers, and clinicians for the development of molecular-targeted therapy of human diseases.

Discusses cutting-edge methodologies and integrated interdisciplinary approaches to the study of post-translational modifications that regulate critical cellular signal pathways Draws together recently identified regulatory mechanisms and biological roles of cell signaling, with recent advances in our understanding of the molecular basis of human diseases Is written by leading experts in the fields of cell signaling and post-translational modifications

Inhalt

Preface.- Part I Novel Analytical Procedures for Signal Transduction.- 1 Phosphoproteomics-based Network Analysis of Cancer Cell Signaling Systems (Hiroko Kozuka-Hata and Masaaki Oyama).- 2 Phos-tag-based Affinity Chromatography Techniques for Enrichment of the Phosphoproteome (Eiji Kinoshita, Emiko Kinoshita-Kikuta, and Tohru Koike).- 3 Visualization of Intracellular Signaling with Fluorescence Resonance Energy Transfer-based Biosensors (Kazuhiro Aoki).- 4 Technology of Wheat Cell-free Based Protein Array for Biochemical Analyses of Protein Kinases and Ubiquitin E3 Ligases (Hirotaka Takahashi, Keiichirou Nemoto, Ramadan Abdelaziz, Atsushi Uematsu, and Tatsuya Sawasaki).- Part II Mathematical Simulation of Signal Transduction.- 5 Potential Roles of Spatial Parameters in the Regulation of NF-B Oscillations, as Revealed by Computer Simulations (Daisuke Ohshima, Jun-ichiro Inoue, and Kazuhisa Ichikawa).- 6 Stochastic Simulation of Stress Granules (Daisuke Ohshima, KyokoArimoto-Matsuzaki, Taichiro Tomida, Mutsuhiro Takekawa, and Kazuhisa Ichikawa).- 7 Temporal Coding of Insulin Signaling (Hiroyuki Kubota and Shinya Kuroda).- Part III Structural Analysis of Signal Transduction.- 8 Structural Biology of Protein Post-transcriptional Modifications and Cellular Signaling (Kazuki Kato, Hiroshi Nishimasu, Ryohei Ishii, Osamu Nureki, and Ryuichiro Ishitani).- 9 Structural Basis for the Signal Initiation by TNF and TNFR (Teruya Nakamura, Yohei Mukai, Yasuo Tsutsumi, and Yuriko Yamagata).- 10 Regulation of NF-B Pathway by Linkage-specific Ubiquitin-binding Domains (Yusuke Sato).- Part IV Regulation of Signal Transduction by Post-translational Modifications and Its Pathogenic Dysregulation.- 11 NF-B Signaling and Lymphoid Malignancies (Shoji Yamaoka).- 12 Ubiquitination-mediated NF-B Regulation in Inflammatory Response (Fuminori Tokunaga).- 13 NF-B Signaling in Osteoclastogenesis (Yuu Taguchi, Jin Gohda, and Jun-ichiro Inoue).- 14 Mitogen-activated Protein Kinase Signaling and Cancer (Mutsuhiro Takekawa and Yuji Kubota).- 15 Critical Roles of the AKT Substrate Girdin in Disease Initiation and Progression (Atsushi Enomoto, Liang Weng, and Masahide Takahashi).- 16 Protein Modifications Pace the Circadian Oscillation of Biological Clocks (Hikari Yoshitane and Yoshitaka Fukada).- 17 Keap1-Nrf2 Regulatory System and Cancer (Keiko Taguchi and Masayuki Yamamoto).- 18 Actin Filament Formation in Myofibrils and Cell Protrusions Regulated by Signal Transduction (Takeshi Endo and Kazunori Takano).- 19 Molecular Signaling and its Pathogenic Alterations in Neuromuscular Junction Formation and Maintenance (Ryo Ueta and Yuji Yamanashi).- 20 Post-translational Modification of Tcf/Lef: New Insights into the Regulation of Wnt/-catenin Signaling (Tohru Ishitani).- BM Index.<p