The idea for this book came from discussions among participants in a symposium on biotechnical applications at the "Pacifichem 89" meeting in Honolulu. It was the majority opinion of this group that a volume dedicated to biotechnical and biomedical applications of PEG chemistry would enhance research and development in this area. Though the book was conceived at the Honolulu meeting, it is not a proceedings of this symposium. Several groups who did not participate in this meeting are repre sented in the book, and the book incorporates much work done after the meeting. The book does not include contributions in all related areas to which PEG chemistry has been applied. Several invited researchers declined to parti.:ipate, and there is not enough space in this single volume to properly cover all submissions. Chapter I-an overview of the topic-discusses in brief applications not given detailed coverage in specifically devoted chapters. The following topics are covered: introduction to and fundamental properties of PEG and derivatives in Chapters 1-3; separations using aqueous polymer two-phase partitioning in Chapters 4-6; PEG-proteins as catalysts in biotechnical applications in Chapters 7 and 8; biomedical applications of PEG-proteins in Chapters 9-13; PEG modified surfaces for a variety of biomedical and biotechnical applications in Chapters 14-20; and synthesis of new PEG derivatives in Chapters 21 and 22.

Inhalt

1. Introduction to Biotechnical and Biomedical Applications of Poly(Ethylene Glycol).- 2. Water Structure of PEG Solutions by Differential Scanning Calorimetry Measurements.- 3. Molecular Simulation of Protein-PEG Interaction.- 4. PEG-Derivatized Ligands with Hydrophobic and Immunological Specificity: Applications in Cell Separation.- 5. Affinity Partitioning in PEG-Containing Two-Phase Systems.- 6. Aqueous Two-Phase Partitioning on an Industrial Scale.- 7. PEG-Modified Protein Hybrid Catalyst.- 8. PEG-Coupled Semisynthetic Oxidases.- 9. Preparation and Properties of Monomethoxypoly(Ethylene Glycol)-Modified Enzymes for Therapeutic Applications.- 10. Suppression of Antibody Responses by Conjugates of Antigens and Monomethoxypoly(Ethylene Glycol).- 11. Toxicity of Bilirubin and Detoxification by PEG-Bilirubin Oxidase Conjugate: A New Tactic for Treatment of Jaundice.- 12. PEG-Modified Hemoglobin as an Oxygen Carrier.- 13. Bovine Collagen Modified by PEG.- 14. Poly(Ethylene Oxide) and Blood Contact: A Chronicle of One Laboratory.- 15. Properties of Immobilized PEG Films and the Interaction with Proteins: Experiments and Modeling.- 16. Protein Adsorption to and Elution from Polyether Surfaces.- 17. Poly(Ethylene Glycol) Gels and Drug Delivery.- 18. PEO-Modified SurfacesIn Vitro, Ex Vivo, and In Vivo Blood Compatibility.- 19. Immobilization of Proteins via PEG Chains.- 20. Polystyrene-Immobilized PEG Chains: Dynamics and Application in Peptide Synthesis, Immunology, and Chromatography.- 21. Use of Functionalized Poly(Ethylene Glycol)s for Modification of Polypeptides.- 22. Synthesis of New Poly(Ethylene Glycol) Derivatives.