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Polyamines

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Recent findings have revolutionized the study of these ubiquitous cellular components, essential for growth and development. Here,... Weiterlesen
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Beschreibung

Recent findings have revolutionized the study of these ubiquitous cellular components, essential for growth and development. Here, leading researchers contribute an extensive collection of up-to-date laboratory techniques that will enhance polyamine study.


Recently, important new findings in the polyamine field and a variety of new experimental systems have revolutionized the study of these ubiquitous cellular components, essential for normal growth and development. In Polyamines: Methods and Protocols, leading researchers contribute an extensive collection of up-to-date laboratory techniques for the further pursuit of polyamine study. The volume delves into vital subjects such as neoplasia studies with animal models and human patients, therapeutic roles for polyamine inhibitors and analogs, polyamine metabolism and oxidative damage, polyamines as regulators of critical ion channels, as well as polyamine transport systems and polyamine-responsive genes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Polyamines: Methods and Protocols provides a key resource for all scientists pursuing the study of this dynamic and significant aspect of cellular biology.

Contains comprehensive and extensive practical coverage of polyamine-related methodologies in a wide variety of subject areas

Features step-by-step protocols, clearly written and readily reproducible

Includes expert tips and key implementation advice to ensure methodological success



Zusammenfassung
The volume delves into vital subjects such as neoplasia studies with animal models and human patients, therapeutic roles for polyamine inhibitors and analogs, polyamine metabolism and oxidative damage, polyamines as regulators of critical ion channels, as well as polyamine transport systems and polyamine-responsive genes.

Inhalt

Part I: Introduction

1. Current Status of the Polyamine Research Field

Anthony E. Pegg and Robert A. Casero, Jr.

Part II: Identification of Polyamine Metabolic Enzymes and Characterization of Polyamine-Regulated Genes and Ion Channels

2. Exploring Polyamine Biosynthetic Diversity through Comparative and Functional Genomics

Anthony J. Michael

3. Characterization of Genes for Polyamine Modulon

Kazuei Igarashi and Keiko Kashiwagi

4. Posttranscriptional Regulation of Gene Expression in Epithelial Cells by Polyamines

Lan Xiao and Jian-Ying Wang

5. Identification, Chemical Synthesis, and Biological Functions of Unusual Polyamines Produced by Extreme Thermophiles

Tairo Oshima, Toshiyuki Moriya, and Yusuke Terui

6. Polyamine Block of Inwardly-Rectifying Potassium Channels

Harley T. Kurata, Wayland W.L. Cheng, and Colin G. Nichols

Part III: Transgenic Models for Study of Polyamine Function

7. Carcinogenesis Studies in Mice with Genetically Engineered Alterations in Polyamine Metabolism

David J. Feith

8. Transgenic Rodents with Altered SSAT Expression as Models of Pancreatitis and Altered Glucose and Lipid Metabolism

Anne Uimari, Mervi T. Hyvönen, Eija Pirinen, and Leena Alhonen

9. Use of (Gyro) Gy and Spermine Synthase Transgenic Mice to Study Functions of Spermine

Xiaojing Wang and Anthony E. Pegg

Part IV: Enzymes Involved in Polyamine Catabolism and Post-Translational Protein Modification

10. A Simple Assay for Mammalian Spermine Oxidase: A Polyamine Catabolic Enzyme Implicated in Drug Response and Disease

Andrew C. Goodwin, Tracy R. Murray-Stewart, and Robert A. Casero, Jr.

11. Characterization, Assay, and Substrate Specificity of Plant Polyamine Oxidases

Panagiotis N. Moschou and Kalliopi A. Roubelakis-Angelakis

12. Assay of Deoxyhypusine Synthase Activity

Edith C. Wolff, Seung Bum Lee, and Myung Hee Park

13. Assay of Deoxyhypusine Hydroxylase Activity

Jong Hwan Park, Edith C. Wolff, and Myung Hee Park

Part V: Regulation of Polyamine Content

14. Identification and Assay of Allosteric Regulators of S-Adenosylmethionine Decarboxylase

Erin K. Willert, Lisa N. Kinch, and Margaret A. Phillips

15. Protocols for Studying Antizyme Expression and Function

Noriyuki Murai, Yasuko Murakami, and Senya Matsufuji

16. Identification, Assay, and Functional Analysis of the Antizyme Inhibitor Family

Chaim Kahana

17. Post-Transcriptional Regulation of Ornithine Decarboxylase

Shannon L. Nowotarski, Sofia Origanti, and Lisa M. Shantz

Part VI: Polyamine Transport and Uptake

18. Identification and Assays of Polyamine Transport Systems in Escherichia coli and Saccharomyces cerevisiae

Keiko Kashiwagi and Kazuei Igarashi

19. Genetic and Biochemical Analysis of Protozoal Polyamine Transporters

Marie-Pierre Hasne and Buddy Ullman

20. Heparan Sulfate Proteoglycan-Mediated Polyamine Uptake

Johanna Welch, Katrin Svensson, Paulina Kucharzewska, and Mattias Belting

21. Polyamine Transport Systems in Mammalian Cells and Tissues

Takeshi Uemura and Eugene W. Gerner

22. Procedures to Evaluate the Importance of Dietary Polyamines

Paul Acheampong, Mary J. Macleod, and Heather M. Wallace

Part VII: Polyamines and Disease

23. Determination of N 1,N 12-Diacetylspermine in Urine: A Novel Tumor Marker

Masao Kawakita, Kyoko Hiramatsu, Mari Yanagiya, Yosuke Doi, and Mieko Kosaka

24. Spermidine/spermine-N 1-Acetyltransferase in Kidney Ischemia Reperfusion Injury

Kamyar Zahedi and Manoocher Soleimani

25. Use of Polyamine Metabolites as Markers for Stroke and Renal Failure

Kazuei Igarashi and Keiko Kashiwagi

26. Methods to Evaluate Alterations in Polyamine Metabolism Caused by Helicobacter pylori Infection

Alain P. Gobert, Rupesh Chaturvedi, and Keith T. Wilson

27. High-Resolution Capillary Gas Chromatography in Combination with Mass Spectrometry for Quantification of Three Major Polyamines in Post-Mortem Brain Cortex

Gary Gang Chen, Laura M. Fiori, Orval A. Mamer, and Gustavo Turecki

28. Spermine Synthase Deficiency Resulting in X-Linked Intellectual Disability (Snyder-Robinson Syndrome)

Charles E. Schwartz, Xaiojing Wang, Roger E. Stevenson, and Anthony E. Pegg

Part VIII: Chemistry and Analysis

29. Methylated Polyamines as Research Tools

Alex R. Khomutov, Janne Weisell, Maxim A. Khomutov, Nikolay A. Grigorenko, Alina R. Simonian, Merja R. Häkkinen, Tuomo A. Keinänen, Mervi T. Hyvönen, Leena Alhonen, Sergey N. Kochetkov, and Jouko Vepsäläinen

30. Fluorescent Substrates for Polyamine Catabolic Enzymes and Transport

Koichi Takao and Akira Shirahata

31. Use of Polyamine Derivatives as Selective Histone Deacetylase Inhibitors

Patrick M. Woster

32. Measurement of Polyamine pKa Values

Ian S. Blagbrough, Abdelkader A. Metwally, and Andrew J. Geall

33. Polyamine Analysis by LC-MS

Merja R. Häkkinen

Produktinformationen

Titel: Polyamines
Untertitel: Methods and Protocols
Editor:
EAN: 9781493958399
ISBN: 1493958399
Format: Kartonierter Einband
Herausgeber: Humana Press
Anzahl Seiten: 540
Gewicht: 1003g
Größe: H254mm x B178mm x T28mm
Jahr: 2016
Auflage: Softcover reprint of the original 1st ed. 2011

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