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Platelets and Megakaryocytes
- Kartonierter Einband
- 424 Seiten
Beschreibung
This comprehensive volume covers techniques for the study of platelet function, approaches for investigating megakaryocyte function, and perspectives on important overall concepts in the field of megakaryocyte and platelet biology.
New techniques to study cell signaling and function can develop at a staggering pace; however, many approaches are as valid today as on the day they were established. Thus, the main aim of Platelets and Megakaryocytes: Volume 3, Additional Protocols and Perspectives is to complement the first two volumes published in 2004 by adding recently developed state of the art techniques. Conveniently divided into three sections, this detailed volume covers techniques to study platelet function, approaches to investigate megakaryocyte function, and perspectives on important overall concepts in the field of megakaryocyte and platelet biology. Written in the highly successful Methods in Molecular Biology™ series format, methods chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Platelets and Megakaryocytes: Volume 3, Additional Protocols and Perspectives adds a wealth of new expertise for the labs of scientists working in this key biological area of study.
Compliments the two previous volumes by supplying fully up-to-date techniques
Includes many detailed protocols that are lab ready and tested
Features expert tips and vital implementation advice to ensure successful results
Inhalt
Part I: Additional Protocols for the Study of Platelet Function
1. Intravital Imaging of Thrombus Formation in Small and Large Mouse Arteries: Experimentally-Induced Vascular Damage and Plaque Rupture In Vivo
Marijke J.E. Kuijpers and Johan W.M. Heemskerk
2. Assessment of Platelet Aggregation Responses In Vivo in the Mouse
Christopher Moore and Michael Emerson
3. Focal Cerebral Ischemia
Stefan Braeuninger, Christoph Kleinschnitz, Bernhard Nieswandt, and Guido Stoll
4. Extracorporeal Assays of Thrombosis
Lina Badimon, Teresa Padro, and Gemma Vilahur
5. Platelet Life Span and Apoptosis
Emma C. Josefsson, Michael J. White, Mark R. Dowling, and Benjamin T. Kile
6. A Live Cell Micro-Imaging Technique to Examine Platelet Calcium Signalling Dynamics Under Blood Flow
Warwick S. Nesbitt, Ian S. Harper, Simone M. Schoenwaelder, Yuping Yuan, and Shaun P. Jackson
7. Platelet Shape Change and Spreading
Joseph E. Aslan, Asako Itakura, Jacqueline M. Gertz, and Owen J.T. McCarty
8. Clot Retraction
Katherine L. Tucker, Tanya Sage, and Jonathan M. Gibbins
9. Visualization and Manipulation of the Platelet and Megakaryocyte Cytoskeleton
Jonathan N. Thon and Joseph E. Italiano
10. Measurement of Platelet Microparticles
Jeffrey I. Zwicker, Romaric Lacroix, Françoise Dignat-George, Barbara C. Furie, and Bruce Furie
11. Assessing Protein Synthesis by Platelets
Hansjörg Schwertz, Jesse W. Rowley, Neal D. Tolley, Robert A. Campbell, and Andrew S. Weyrich
12. A Rapid and Efficient Platelet Purification Protocol for Platelet Gene Expression Studies
Stefan Amisten
Part II: Additional Protocols for the Study of Megakaryocyte Function
13. Characterization of Megakaryocyte Development in the Native Bone Marrow Environment
Anita Eckly, Catherine Strassel, Jean-Pierre Cazenave, François Lanza, Catherine Léon, and Christian Gachet
14. Culture of Murine Megakaryocytes and Platelets from Fetal Liver and Bone Marrow
Harald Schulze
15. In Vitro Generation of Megakaryocytes and Platelets from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells
Naoya Takayama and Koji Eto
16. Megakaryocyte and Platelet Production from Human Cord Blood Stem Cell s
Amélie Robert, Valérie Cortin, Alain Garnier, and Nicolas Pineault
17. Culture of Megakaryocytes and Platelets from Subcutaneous Adipose Tissue and a Preadipocyte Cell Line
Yumiko Matsubara, Mitsuru Murata, and Yasuo Ikeda
18. Purification of Native Bone Marrow Megakaryocytes for Studies of Gene Expression
Gwen Tolhurst, Richard N. Carter, Nigel Miller, and Martyn P. Mahaut-Smith
19. Assessment of Megakaryocyte Migration and Chemotaxis
Alexandra Mazharian
Part III: Perspectives on the Study of Platelet and Megakaryocyte Function
20. Megakaryopoiesis and Thrombopoiesis: An Update on Cytokines and Lineage Surface Markers
Ming Yu and Alan B. Cantor
21. Using Zebrafish (Danio rerio) to Assess Gene Function in Thrombus Formation
Christopher M. Williams and Alastair W. Poole
22. Platelet Receptor Shedding
Elizabeth E. Gardiner, Mohammad Al-Tamimi, Robert K. Andrews, and Michael C. Berndt
23. Endogenous Inhibitory Mechanisms and the Regulation of Platelet Function
Chris I. Jones, Natasha E. Barrett, Leonardo A. Moraes, Jonathan M. Gibbins, and Denise E. Jackson
24. Platelet Proteomics: State of the Art and Future Perspective
Yotis Senis and Ángel García
Produktinformationen
| Titel: | Platelets and Megakaryocytes |
| Untertitel: | Volume 3, Additional Protocols and Perspectives |
| Editor: | |
| EAN: | 9781493958474 |
| ISBN: | 149395847X |
| Format: | Kartonierter Einband |
| Herausgeber: | Humana Press |
| Anzahl Seiten: | 424 |
| Gewicht: | 793g |
| Größe: | H254mm x B178mm x T22mm |
| Jahr: | 2016 |
| Untertitel: | Englisch |
| Auflage: | Softcover reprint of the original 1st ed. 2012 |
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