Plant Signal Transduction

This fully updated volume reflects the spectacular advances in our knowledge of signal transduction pathways with a selection of 'classic' as well as newly developed approaches. These detailed approaches expand into the fields of molecular biology, biochemistry, physiology, cell biology, genetics, and genomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and up-to-date, Plant Signal Transduction: Methods and Protocols, Second Edition serves as an ideal guide for researchers exploring the vast array of signals produced by plants to ensure their survival.

Includes updated and cutting-edge methods and protocols for studying plant signal transduction Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

Suppressor Screens in Arabidopsis.- Quantitative Reverse Transcription-qPCR-Based Gene Expression Analysis in Plants.- DNA-Binding Factor Target Identification by Chromatin Immunoprecipitation (ChIP) in Plants.- Identification of Genes Responsible for Natural Variation in Volatile Content Using Next Generation Sequencing Technology.- Micro-Tom Tomato as an Alternative Plant Model System: Mutant Collection and Efficient Transformation.- Culture of the Tomato Micro-Tom Cultivar in Greenhouse.- Virus-Induced Gene Silencing as a Tool to Study Tomato Fruit Biochemistry.- A Simplified and Rapid Method for the Isolation and Transfection of Arabidopsis Leaf Mesophyll Protoplasts for Large-Scale Applications.- Preparation of Epidermal Peels and Guard Cell Protoplasts for Cellular, Electrophysiological, and -Omics Assays of Guard Cell Function.- Application of Variable Angle Total Internal Reflection Fluorescence Microscopy to Investigate Protein Dynamics in Intact Plant Cells.- Immunoprecipitation of Plasma Membrane Receptor-Like Kinases for Identification of Phosphorylation Sites and Associated Proteins.- Yeast Three-Hybrid System for the Detection of Protein-Protein Interactions.- Cautions in Measuring In Vivo Interactions Using FRET and BiFC in Nicotiana benthamiana .- Analysis of Protein-Lipid Interactions Using Purified C2 Domains.- Assessing Kinase Activity in Plants with In-Gel Kinase Assays.- Analyses of Plant UDP-Dependent Glycosyltransferases to Identify Their Volatile Substrates Using Recombinant Proteins.- Competitive Index: Mixed Infection-Based Virulence Assays for Genetic Analysis in Pseudomonas syringae -Plant Interactions.- Simplified Assays for Evaluation of Resistance to Alternaria brassicicola and Turnip Mosaic Virus.- Simultaneous Determination of Plant Hormones by GC-TOF-MS.