This volume provides lab-oriented protocols to deal the various plant microbiome engineering approaches in a lucid and simple manner. Chapters are divided into four section detailing plant associated microbiomes, single cell genomics, whole community metagenomics, metabolic network monitoring and advanced methods in plant microbiome engineering. Written in the format of the Methods and Protocols in Food Science series, the chapters include an introduction to the respective topic, list necessary materials and reagents, detail well-established and validated methods for readily reproducible laboratory protocols and contain notes on how to avoid or solve typical problems.

Authoritative and cutting-edge, Plant Microbiome Engineering aims to provide well-established protocols and procedures largely used by both academics and industrials.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Collection and processing of soil, rhizosphere, root tissue from crop field and forests.- Collection and processing of bacteria from plant Vascular tissues.- Collection and processing of plant fungal endophytes from shrubs.- Collection And Processing Of Microalgae From Coralloid Roots Of Cycads.- Collection and processing of plant endophytes from large woody plants.- Collection and processing of halotolerant plant growth-promoting bacteria from plant roots and adjacent areas.- Detection of Plant Growth Promoting Bacteria from Soil.- Detection of plant growth promoting cyanobacterial community from soil and root.- Detection of plant growth promoting actinobacterial community from soil.- Detection of plant growth promoting fungal community from soil.- Detection of microalgal community from soil and plant root.- Determine the Symbiotic nitrogen fixation bacterial community structure in legume crops.- Determine the Symbiotic nitrogen fixation bacterial community structure in Casuarina sp..- Detection of endophytic bacterial community structure in medicinal plant.- Rapid Detection of Plant Associated Bacteria by MALDI-TOF Mass Spectrometry.- Rapid Detection of Plant-associated Fungi by MALDI-TOF Mass Spectrometry.- Rapid detection of plant-associated microalgae by MALDI-TOF mass spectrometry.- Nucleic Acid-based Rapid Detection of Plant-associated Cyanobacteria.- Detection of plant associated fungi by nucleic acid based technique.- Nucleic Acid-Based Rapid Detection of Microalgae association in Plant.- Isolation of Genomic DNA from plant growth promoting Pseudomonas species.- Isolation of genomic DNA from plant growth promoting Streptomyces sp..- Isolation of Genomic DNA from Plant Growth Promoting Cyanobacteria.- ISOLATION OF GENOMIC DNA FROM MICROALGAE.- Separation of genomic DNA by agarose gel electrophoresis.- Isolation of metagenomic DNA from rhizosphere soil.- Isolation of metagenomic DNA from plant roots.- isolation of metagenomic DNA from root nodules.- Quantification of metagenomic DNA.- COLONY PCR FOR RAPID DETECTION AND IDENTIFICATION OF MICROALGAE.- Quantitative PCR for monitoring the plant growth promoting microalgae from rice field.- Multivariate Analysis of Plant microbiome diversity using Paleontological Statistics (PAST).- DNA extraction, amplification and high-throughput sequencing of the 18S and ITS regions of endophytic fungi.- Nextera DNA flex library preparation.- Illumina Next Generation Sequencing of Metagenome.- Alpha diversity analysis using Shannon index for each species in the rhizosphere soil.- Microbiome analyses of Metagenome using USEARCH.- 16S rRNA microbiome analysis using QIIME.- Microbiome Analysis of Metagenome using R studio.- Omics tools in plant microbiome interactions.- Inference and Analysis of SPIEC-EASI Microbiome Networks.- Rhizosphere meta transcriptomics.- Phyto microbiomes engineering using clustered regularly interspaced short palindromic repeats (CRISPR).- Transformation techniques for engineering microbiome bacterial species.- Conjugation techniques for engineering microbiome bacterial species.- Plant growth promoting functional analysis of Whole metagenome sequence of bacteria.- Plant growth promoting functional analysis of Whole metagenome sequence of Actinobacteria.- Plant growth promoting functional analysis of Whole metagenome sequence of fungi.- In Situ Phyto microbiome Engineering.- Inoculation of microorganisms into the soil and rhizosphere.- Inoculation of microbes into seeds/ seedlings.- Direct injection of microbes into tissues / wounds.- Microbiome Engineering for Plant Growth and Improvement.- Phyto-Microbiome Engineering For Crop Growth And Resilience.- Plant Microbiome Engineering to improve plant growth promotion and biocontrol.- Microbiome engineering in Chromium Phytoremediation.- Measurement of Arsenic-Tolerance Index of Fungal Strains as an Approach to Mitigating Arsenic toxicity in Sustainable Agriculture.- Plant Microbiome Engineering to improve Stress tolerance.- Plant Microbiome Engineering to improve Drought Stress tolerance.- Genome Engineering Of Chlorella Sp..- Genome Engineering of Frankia sp..- Bacillus genome engineering using Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR).