Plant Gametogenesis

This volume provides protocols and methods on techniques to study plant gametogenesis. Chapters are divided into four sections covering omics, cytological, molecular approaches, plant transformation, genome editing, bioinformatics, and data analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Plant Gametogenesis: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

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Measurement of flower metabolite concentrations using Gas-Chromatography Mass Spectrometry and High-Performance Liquid Chromatography Mass Spectrometry.- Proteomic-, phosphoproteomic- and Acetylomic-based mass spectrometry to identify tissue-specific protein complexes and phosphorylation in plant gametogenesis.- Identifying small RNAs and analyzing their association with gene expression using isolated Arabidopsis male meiocytes.- Genotyping-by-sequencing to map meiotic crossovers in plants.- Interrogating global chromatin interaction network by high-throughput chromosome conformation capture (Hi-C) in plants.- Cytogenetic techniques for analysing meiosis in hexaploidy bread wheat.- Super-resolution chromatin visualisation by combined fluorescence in situ hybridisation and Structured Illumination Microscopy in Solanum lycopersicum.- Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy.- Bimolecular Fluorescence Complementation to Test for Protein-Protein Interactions and To Uncover Regulatory Mechanisms During Gametogenesis.- Quantifying meiotic crossover recombination in Arabidopsis lines expressing fluorescent reporters in seeds using SeedScoring pipeline for CellProfile.- Generation of mutants by combined treatment of physical and chemical mutagens in rice.- Generation of mutant plants by Gamma Ray exposure and development of low-cost TILLING population in chickpea ( Cicer arietinum L.).- Efficient generation of CRISPR/Cas9-based mutants supported by fluorescent seed selection in different Arabidopsis accessions.- Generation of deletion lines in Allohexaploid Bread Wheat.- Post-transcriptional Gene Silencing Using Virus-Induced Gene Silencing to Study Plant Gametogenesis in Tomato.- Protocols for in vivo doubled haploid (DH) technology in maize breeding: From haploid inducer Development to Haploid Genome Doubling.- Estimation of heterografting associated DNA methylation changes in tree crops by MSAP analysis.- In vitro fertilization system using wheat gametes by electric fusion.- High-throughput fluorescent pollen tetrad analysis using DeepTetrad.- Barley ( Hordeum vulgare ) anther and meiocyte RNA Sequencing: Mapping Sequencing reads and downstream data analyses.- Profiling m6A RNA modifications in low amounts of plant cells using Maize Meiocytes.- Assembling Plant Genomes with Long-Read Sequencing.- Mapping-by-sequencing of point and insertional mutations with Easymap.- Characterization of the different levels of variation in 45S rRNA genes.