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Plant Cytogenetics

  • Fester Einband
  • 212 Seiten
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This volume covers a range of methods used in plant cytogenetics, beginning with basic analysis of chromosomes and visualizing gen... Weiterlesen
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Beschreibung

This volume covers a range of methods used in plant cytogenetics, beginning with basic analysis of chromosomes and visualizing gene locations, to manipulating and dissecting chromosomes, and then focusing on less understood features of chromosomes such as recombination initiation sites and epigenomic marks. The methods described in Plant Cytogenetics: Methods and Protocols build on each other and provide, those new to the field, with a comprehensive platform to support their research endeavours, while also introducing advanced techniques to experienced researchers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, Plant Cytogenetics: Methods and Protocols, is a valuable resource for anyone who is interested in the diverse and wonderfully complex field of cytogenetics.



Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts



Autorentext
Shahryar Kianian Research Leader USDA-ARS Cereal Disease Laboratory University of Minnesota St. Paul, MN 55108 
Email: Shahryar.Kianian@ars.usda.gov

Penny M Avoles Kianian Alderman Hall, St. Paul, MN 55108  
Email:P.Kianian@gmail.com 

Klappentext

This volume covers a
range of methods used in plant cytogenetics, beginning with basic analysis of
chromosomes and visualizing gene locations, to manipulating and dissecting
chromosomes, and then focusing on less understood features of chromosomes such
as recombination initiation sites and epigenomic marks. The methods described
in Plant Cytogenetics: Methods and Protocols build on each other and
provide, those new to the field, with a comprehensive platform to support their
research endeavours, while also introducing advanced techniques to experienced
researchers. Written in the highly successful Methods in Molecular Biology series
format, chapters include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting
edge and thorough, Plant Cytogenetics: Methods and Protocols, is a
valuable resource for anyone who is interested in the diverse and wonderfully
complex field of cytogenetics.



Inhalt
1. C-Banding of Plant ChromosomesEric N. Jellen2. Chromosome Painting by GISH and Multi-Color FISHSteven S. Xu, Zhao Liu, Qijun Zhang, Zhixia Niu, Chao-Chien Jan, and Xiwen Cai3. Fluorescent In Situ Hybridization on Extended Chromatin Fibers for High-Resolution Analysis of Plant ChromosomesDaryna Dechyeva and Thomas Schmidt4. Tyramide Signal Amplification: Fluorescence In Situ Hybridization for Identifying Homologous ChromosomesAraceli Fominaya, Yolanda Loarce, Juan M. González, and Esther Ferrer5. Localization of Low-Copy DNA Sequences on Mitotic Chromosomes by FISHMiroslava Karafiátová, Jan Barto, Jaroslav Doleel6. Immunolabelling and In Situ Labelling of Isolated Plant Interphase NucleiAli Pendle and Peter Shaw7. Manipulation of Homologous Chromosome Recombination in WheatAdam J. Lukaszewski8. Dissecting Plant Chromosomes by the Use of Ionizing RadiationPenny M.A. Kianian, Katie L. Liberatore, Marisa Miller, Justin B. Hegstad, and Shahryar F. Kianian9. Optical Nano-Mapping and Analysis of Plant GenomesMing-Cheng Luo, Karin R. Deal, Armond Murray, Tingting Zhu, Alex R. Hastie, Will Stedman, Henry Sadowski, and Michael Saghbini10. Flow Sorting Plant ChromosomesJan Vrána, Petr Cápal, Jarmila íhalíková, Marie Kubaláková, and Jaroslav Doleel11. Construction of BAC Libraries from Flow-Sorted ChromosomesJan afá, Hana imková, and Jaroslav Doleel12. The Chromosome Microdissection and Microcloning TechniqueYing-Xin Zhang, Chuan-Liang Deng, and Zan-Min Hu13. Immunolocalization on Whole Anther Chromosome Spreads for Male MeiosisStefanie Dukowic-Schulze, Anthony Harris, and Changbin Chen14. Mapping Recombination Initiation Sites Using Chromatin ImmunoprecipitationYan He, Minghui Wang, Qi Sun, and Wojciech P. Pawlowski15. Chromatin Immunoprecipitation to Study Plant EpigenomeZidian Xie and Gernot Presting

Produktinformationen

Titel: Plant Cytogenetics
Untertitel: Methods and Protocols
Editor:
EAN: 9781493936205
ISBN: 1493936204
Format: Fester Einband
Herausgeber: Springer New York
Anzahl Seiten: 212
Gewicht: 605g
Größe: H260mm x B183mm x T17mm
Jahr: 2016
Auflage: 1st ed. 2016

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