This fourth edition provides new and updated protocols on plant cell, tissue, and organ cultures. Chapters are divided into five parts that cover topics from general methodologies, statistical analysis and contamination control, highly specialized techniques, and laborious process of measuring the epigenetics changes in tissue cultures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Cell Culture Protocols, Fourth Edition aims to serve both professionals and novices with its guide to the most common and applicable techniques and methods for plant tissue and cell culture.

Includes cutting-edge methods protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
An Introduction to Plant Tissue Culture: Advances and Perspectives.- Micropropagation in the XXI Century.- Cellular and Morpho-histological Foundations of in vitro Plant Regeneration .- Bacterial Endophytes in Plant Tissue Culture: Mode of Action, Detection, and Control .- Digital Photography as a Tool of Research and Documentation in Plant Tissue Culture.- Selection of Molecular Markers for the Estimation of Somaclonal Variation.- Plant Tissue Culture. A Battle Horse in the Genome Editing Using CRISPR/Cas9.- Micropropagation of Agave Species.- Protocol for the Micropropagation of Coconut from Plumule Explants.- Micropropagation of Yucca Species.- Auxin Immunolocalization in Coffea canephora Tissues.- Somatic Embryogenesis in Common Bean Phaseolus vulgaris L..- Induction of Somatic embryogenesis in Jatropha curcas .- In Vitro Proliferation of Female Buds for Induction of Somatic Embryogenesis from False Horn Plantain (AAB, Curraré) .- Somatic Embryogenesis in Theobroma cacao L..- Somatic Embryogenesis of Quercus suber L. from Immature Zygotic Embryos.- Cryotherapy: a Novel Method for Virus Eradication in Economically Important Plant Species.- Cryopreservation of Pineapple Shoot Tips by the Droplet Vitrification Technique.- Cryopreservation of Pollen Grains of Pineapple and Other Bromeliads.- Application of in Casa Pollination and Embryo Rescue Techniques for Breeding of Agave Species.- Haploid and Doubled Haploid Plant Production in Carrot Using Induced Parthenogenesis and Ovule Excision in Vitro.- Using Flow Cytometry Analysis in Plant Tissue Culture Derived Plants.- Procedure for Estimating the Tolerance and Accumulation of Heavy Metals Using Plant Cell Cultures.- Proteomics as a Tool to Study Molecular Changes During Plant Morphogenesis in Vitro.- Proteomic Analysis of Non-Model Plant Tissues Using Phenol Extraction, Two-Dimensional Electrophoresis and MALDI Mass Spectrometry.- Chromatin Immunoprecipitation (ChiP) Protocol for the Analysis of Gene Regulation by Histone Modifications in Agave angustifolia Haw.- Transcription Factors: Their Role in the Regulation of Somatic Embryogenesis in Theobroma cacao L. and other Species.- MicroRNA Expression and Regulation During Maize Somatic Embryogenesis.- Elaboration of Transcriptome during the Induction of Somatic Embryogenesis.- Induction of Specialized Metabolism in in vitro Cultures of Capsicum chinense Jacq..- Analysis of Terpenoid Indole Alkaloids, Carotenoids, Phytosterols, and NMR-Based Metabolomics for Catharanthus roseus Cell Suspension Cultures..- Transformed Root Culture: from Genetic Transformation to NMR-based Metabolomics .- Genetic Transformation of Pentalinon andrieuxii Tissue Cultures.- Appendix A: The Components of the Culture Media. <p