This volume presents a comprehensive collection of protocols and techniques commonly used in research on plant-biotic interactions. Chapters guide readers through general plantpathogen interactions, RNA interference (RNAi) in Okra , spot inoculation of begomoviruses, natural transmission, RNA viruses, enzyme-linked immunosorbent, reverse transcription polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (RT-LAMP), northern blot hybridization, and next-generation sequencing (NGS) for in-depth analysis of viral populations and mutation patterns. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights ** tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Biotic Stress: Methods and Protocols aims to be comprehensive guide for ** researchers in the field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Plant Biotic Stress: Tools and Techniques for Crop Protection.- Investigating the Involvement of Target Protein in Plant-Pathogen Interactions through Ectopic Expression in Arabidopsis A detailed methodology.- RNA interference strategy for the development of okra (Abelmoschus esculentus L. Moench) lines resistant to Yellow vein mosaic virus.- Spot inoculation of begomovirus by single whitefly (Bemisia tabaci) transmission in mungbean using microcage.- One-Step RT-LAMP: A Method for Closed-Tube Colorimetric Detection of RNA Viruses in Plants.- Detection of Sesame Phyllody Disease and Its Transmitting Vectors.- A method for isolation and transfection of Arabidopsis protoplast for sucrose feeding assay.- Twitching motility dynamics in the wilt-causing phytopathogen Ralstonia solanacearum.- A blotting paper technique for the screening of chickpea genotypes against dry root rot disease.- CRISPR-mediated gene editing for inducing thermo-sensitive genic male sterility and sheath blight resistance in rice.- A Seedling Root Dip-based Technique to Screen Chickpea for Resistance to Fusarium Wilt Disease.- Oligo-dT Anchored cDNA SCoT: An Innovative Approach for Deciphering Fusarium wilt Resistance-Related Differential Gene Expression in Chickpea.- Extracellular acidification assay to evaluate the effectiveness of antifungal agents on the pathogenicity of Macrophomina phaseolina.- A guide to culturing, maintenance and leaf inoculation methods for rapid screening and quantification of Sclerotinia sclerotiorum infection in mustard.- Differential-display reverse transcription-PCR for unraveling the molecular mechanisms in foot rot (Phytophthora capsici sp. nov.) tolerance in black pepper (Piper nigrum L.).- Estimation of foliar volatiles emitted by mycorrhizal colonized blackgram (Vigna mungo L) infested with Spodoptera litura.- Development of an Agrobacterium tumefaciens-mediated in planta transformation strategy in pigeonpea for resistance to Helicoverpa armigera.- CRISPR/Cas9 mediated genome editing in the management of oriental fruit fly, Bactrocera dorsalis (Hendel) (Tephritidae: Diptera).- A novel approach using SSR markers for identifying and breeding Bruchid pest resistant black gram (Vigna mungo L. Hepper) genotypes.- RNAi-based approach as a promising tool for management of leafhopper (Amrasca biguttula biguttula) in cotton.- Germplasm evaluation in chickpea for resistance to plant parasitic nematodes.- Novel method for rapid screening of chickpea for combined dry root rot disease and osmotic stress.