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Pichia Protocols

  • Kartonierter Einband
  • 268 Seiten
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This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. The volume fully updates a... Weiterlesen
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This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. The volume fully updates and expands upon the first edition, and focuses primarily on information that has come to light since its original publication.

All protocols are tested and readily and reproducible

Updates and expands upon the first volume

Presents the basics of Pichia pastoris and describes available vectors and host strains for expression


The use of Pichia Pastoris as a recombinant protein system has increased greatly in popularity over the years. In Pichia Protocols 2nd Edition, the author focuses on new information that has come to light in recent years but repeats key basic methods so that researchers are not absolutely dependent on having both editions in order to utilize the system. The chapters in this indispensable laboratory aid cover a wide range of topics associated with Pichia Pastoris. The use of fermentors to grow Pichia Pastoris and good advice on how to go about the initial processing of material from these cultures is discussed. An extensive discussion on how to formally analyze mutations in essential P. pastoris genes is also presented. The final chapters are for cell biologists who are for cell biologists using the Pichia Pastoris system to investigate protein interactions, or to localize proteins in yeast cells. Pichia Protocols 2nd Edition, is an essential tool for researchers working with the Pichia Pastoris system

Table of Contents Chapter 1 - Introduction [REVIEW CHAPTER] Chapter 2 - Vectors and strains for expression [REVIEW CHAPTER] Chapter 3 - DNA-Mediated Transformation Chapter 4 - Rational design and optimization of fed-batch and continuous fermentations Chapter 5 - Overexpression of a hookworm secretory protein saturates secretory capacity Chapter 6 - Initial processing and purification strategies Chapter 7 - Rapid screening of chromatography resins for the purification of proteins Chapter 8 - Characterization of O-linked saccharides on glycoproteins Chapter 9 - Modification of the N-glycosylation pathway to produce homogeneous human-like glycans using GlycoSwitch plasmids Chapter 10 - N-linked glycan characterization of heterologous proteins Chapter 11 - Heavy labeling of recombinant proteins Chapter 12 - Selenomethionine labeling of recombinant proteins Chapter 13 - Deuterium labeling of proteins Chapter 14 - Classical genetic analysis Chapter 15 - Identification of pexophagy genes by Restriction Enzyme-Mediated Integration (REMI) Chapter 16 - Characterization of protein-protein interactions Chapter 17 - Localization of proteins and organelles using fluorescence microscopy Chapter 18 - Fluorescence microscopy and thin-section electron microscopy


Titel: Pichia Protocols
EAN: 9781617375637
ISBN: 978-1-61737-563-7
Format: Kartonierter Einband
Herausgeber: Springer, Berlin
Genre: Biologie
Anzahl Seiten: 268
Gewicht: 434g
Größe: H229mm x B229mm x T152mm
Jahr: 2010
Untertitel: Englisch
Auflage: 2nd Edition.

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