Photosynthesis

This user-friendly book provides a range of classical and modern techniques for the study of photosynthesis in a manner accessible to a broad spectrum of researchers. Broken into four sections, it explores the measurement of physiological photosynthetic parameters, quantifying photosynthetic enzyme abundance and catalytic activity, visualizing cellular and sub-cellular phenotypes, and photosynthesis-inspired energy generation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Photosynthesis: Methods and Protocols aims to aid scientists working toward exciting developments in the understanding and application of photosynthesis.

Includes cutting-edge methods and protocols for the study of photosynthesis Provides step-by-step detail essential for the best results Contains key notes and implementation advice from the experts

Inhalt

Survey of Tools for Measuring In Vivo Photosynthesis.- Photosynthetic Gas Exchange in Land Plants at the Leaf Level.- Design and Use of a Digitally-Controlled Device for Accurate, Multiplexed Gas Exchange Measurements of the Complete Foliar Parts of Plants.- Measuring Canopy Gas Exchange Using CAnopy Photosynthesis and Transpiration Systems (CAPTS).- Light-Response Curves in Land Plants.- Chlorophyll Fluorescence on the Fast Timescale.- Sub-Saturating Multiphase Flash Irradiances to Estimate Maximum Fluorescence Yield.- Chlorophyll Fluorescence Imaging.- Measurement of O 2 Uptake and Evolution in Leaves In Vivo Using Stable Isotopes and Membrane Inlet Mass Spectrometry.- Using Carbon Stable Isotopes to Study C 3 and C 4 Photosynthesis: Models and Calculations.- Liquid-Phase Measurements of Photosynthetic Oxygen Evolution.- Quantification of Photosynthetic Enzymes in Leaf Extracts by Immunoblotting.- Extraction of Rubisco to Determine Catalytic Constants.- Spectrophotometric Determination of Rubisco Activity and Activation State in Leaf Extracts.- Creating Leaf Cell Suspensions for Characterization of Mesophyll and Bundle Sheath Cellular Features.- Determining the Subcellular Localization of Fluorescently-Tagged Proteins Using Protoplasts Extracted from Transiently-Transformed Nicotiana benthamiana Leaves.- 3D Clearing and Molecular Labeling in Plant Tissues.- Evaluation of Lipids for the Study of Photosynthetic Membranes.- Click Methodology for the Functionalization of Water Oxidation Catalyst Iridium Oxide Nanoparticles with Hydrophobic Dyes for Artificial Photosynthetic Constructs.- Biophotovoltaics: Design and Study of Bio-Electrochemical Systems for Biotechnological Applications and Metabolic Investigation.