Informationen zum Autor Philip N. Bartlett is Head of the Electrochemistry Section, Deputy Head of Chemistry for Strategy, and Associate Dean for Enterprise in the Faculty of Natural and Environmental Sciences at the University of Southampton. He received his PhD from Imperial College London and was a Lecturer at the University of Warwick and a Professor for Physical Chemistry at the University of Bath, before moving to his current position. His research interests include bioelectrochemistry, nanostructured materials, and chemical sensors. Klappentext Bioelectrochemistry: Fundamentals, Experimental Techniques and Application , covers the fundamental aspects of the chemistry, physics and biology which underlie this subject area. It describes some of the different experimental techniques that can be used to study bioelectrochemical problems and it describes various applications of biolelectrochemisty including amperometric biosensors, immunoassays, electrochemistry of DNA, biofuel cells, whole cell biosensors, in vivo applications and bioelectrosynthesis. By bringing together these different aspects, this work provides a unique source of information in this area, approaching the subject from a cross-disciplinary viewpoint. Zusammenfassung This two-volume work provides a modern view of the field of bioelectrochemistry. Volume 1 deals with fundamental aspects of the chemistry! physics and biology! which underlie the subject area. Inhaltsverzeichnis List of Contributors. Preface. 1 Bioenergetics and Biological Electron Transport (Philip N. Bartlett). 1.1 Introduction. 1.2 Biological Cells. 1.3 Chemiosmosis. 1.3.1 The Proton Motive Force. 1.3.2 The Synthesis of ATP. 1.4 Electron Transport Chains. 1.4.1 The Mitochondrion. 1.4.2 The NADH-CoQ Reductase Complex. 1.4.3 The Succinate-CoQ Reductase Complex. 1.4.4 The CoQH2-Cyt c Reductase Complex. 1.4.5 The Cyt c Oxidase Complex. 1.4.6 Electron Transport Chains in Bacteria. 1.4.7 Electron Transfer in Photosynthesis. 1.4.8 Photosystem II. 1.4.9 Cytochrome bf Complex. 1.4.10 Photosystem I. 1.4.11 Bacterial Photosynthesis. 1.5 Redox Components. 1.5.1 Quinones. 1.5.2 Flavins. 1.5.3 NAD(P)H. 1.5.4 Hemes. 1.5.5 Iron-Sulfur Clusters. 1.5.6 Copper Centres. 1.6 Governing Principles. 1.6.1 Spatial Separation. 1.6.2 Energetics: Redox Potentials. 1.6.3 Kinetics: Electron Transfer Rate Constants. 1.6.4 Size of Proteins. 1.6.5 One-Electron and Two-Electron Couples. 1.7 ATP Synthase. 1.8 Conclusion. References. 2 Electrochemistry of Redox Enzymes (James F. Rusling, Bingquan Wang and Sei-eok Yun). 2.1 Introduction. 2.1.1 Historical Perspective. 2.1.2 Examples of Soluble Mediators. 2.1.3 Development of Protein-Film Voltammetry and Direct Enzyme Electrochemistry. 2.2 Mediated Enzyme Electrochemistry. 2.2.1 Electron Mediation. 2.2.2 Wiring with Redox Metallopolymer Hydrogels. 2.2.3 Wiring with Conducting Polymers. 2.2.4 NAD(P)þ/NAD(P)H Dependent Enzymes. 2.2.5 Regeneration of NAD(P)H from NAD(P)þ. 2.2.6 Regeneration of NAD(P)þ from NAD(P)H. 2.3 Direct Electron Transfer between Electrodes and Enzymes. 2.3.1 Enzymes in Solution. 2.3.2 Enzyme-Film Voltammetry: Basic Theory. 2.3.3 Adsorbed and Coadsorbed Enzyme Monolayers. 2.3.4 Self-Assembled Monolayers and Covalently Attached Enzymes. 2.3.5 Enzymes on Carbon Nanotube Electrodes. 2.3.6 Enzymes in Lipid Bilayer Films. 2.3.7 Polyion Films and Layer-by-Layer Methods. 2.4 Outlook for the Future. Acknowledgements. References. 3 Biological Membranes and Membrane Mimics (Tibor...

Autorentext

Philip N. Bartlett is Head of the Electrochemistry Section, Deputy Head of Chemistry for Strategy, and Associate Dean for Enterprise in the Faculty of Natural and Environmental Sciences at the University of Southampton. He received his PhD from Imperial College London and was a Lecturer at the University of Warwick and a Professor for Physical Chemistry at the University of Bath, before moving to his current position. His research interests include bioelectrochemistry, nanostructured materials, and chemical sensors.

Klappentext

Bioelectrochemistry: Fundamentals, Experimental Techniques and Application, covers the fundamental aspects of the chemistry, physics and biology which underlie this subject area. It describes some of the different experimental techniques that can be used to study bioelectrochemical problems and it describes various applications of biolelectrochemisty including amperometric biosensors, immunoassays, electrochemistry of DNA, biofuel cells, whole cell biosensors, in vivo applications and bioelectrosynthesis. By bringing together these different aspects, this work provides a unique source of information in this area, approaching the subject from a cross-disciplinary viewpoint.

Zusammenfassung
This two-volume work provides a modern view of the field of bioelectrochemistry. Volume 1 deals with fundamental aspects of the chemistry, physics and biology, which underlie the subject area.

Inhalt

List of Contributors. Preface.

1 Bioenergetics and Biological Electron Transport (Philip N. Bartlett).

1.1 Introduction.

1.2 Biological Cells.

1.3 Chemiosmosis.

1.3.1 The Proton Motive Force.

1.3.2 The Synthesis of ATP.

1.4 Electron Transport Chains.

1.4.1 The Mitochondrion.

1.4.2 The NADH-CoQ Reductase Complex.

1.4.3 The Succinate-CoQ Reductase Complex.

1.4.4 The CoQH2-Cyt c Reductase Complex.

1.4.5 The Cyt c Oxidase Complex.

1.4.6 Electron Transport Chains in Bacteria.

1.4.7 Electron Transfer in Photosynthesis.

1.4.8 Photosystem II.

1.4.9 Cytochrome bf Complex.

1.4.10 Photosystem I.

1.4.11 Bacterial Photosynthesis.

1.5 Redox Components.

1.5.1 Quinones.

1.5.2 Flavins.

1.5.3 NAD(P)H.

1.5.4 Hemes.

1.5.5 Iron-Sulfur Clusters.

1.5.6 Copper Centres.

1.6 Governing Principles.

1.6.1 Spatial Separation.

1.6.2 Energetics: Redox Potentials.

1.6.3 Kinetics: Electron Transfer Rate Constants.

1.6.4 Size of Proteins.

1.6.5 One-Electron and Two-Electron Couples.

1.7 ATP Synthase.

1.8 Conclusion.

References.

2 Electrochemistry of Redox Enzymes (James F. Rusling, Bingquan Wang and Sei-eok Yun).

2.1 Introduction.

2.1.1 Historical Perspective.

2.1.2 Examples of Soluble Mediators.

2.1.3 Development of Protein-Film Voltammetry and Direct Enzyme Electrochemistry.

2.2 Mediated Enzyme Electrochemistry.

2.2.1 Electron Mediation.

2.2.2 Wiring with Redox Metallopolymer Hydrogels.

2.2.3 Wiring with Conducting Polymers.

2.2.4 NAD(P)þ/NAD(P)H Dependent Enzymes.

2.2.5 Regeneration of NAD(P)H from NAD(P)þ.

2.2.6 Regeneration of NAD(P)þ from NAD(P)H.

2.3 Direct Electron Transfer between Electrodes and Enzymes.

2.3.1 Enzymes in Solution.

2.3.2 Enzyme-Film Voltammetry: Basic Theory.

2.3.3 Adsorbed and Coadsorbed Enzyme Monolayers.

2.3.4 Self-Assembled Monolayers and Covalently Attached Enzymes.

2.3.5 Enzymes on Carbon Nanotube Electrodes.

2.3.6 Enzymes in Lipid Bilayer Films.

2.3.7 Polyion Films and Layer-by-Layer Methods.

2.4 Outlook for the Future.

Acknowledgements.

References.

3 Biological Membranes and Membrane Mimics (Tibor Hianik).

3.1 Introduction.

3.2 Membrane Structure and Composition.

3.2.1 Membrane Structure.

3.2.2 Membrane Lipids.

3.2.3 Membrane Proteins.

3.3 Models of Membrane Structure.

3.3.1 Lipid Monolayers.

3.3.2 Bilayer Lipid Membranes (BLM).

3.3.3 Supported Bilayer Lipid Membranes.

3.3.4 Liposomes.

3.4 Ordering, Conformation and Molecular Dynamics of Lipid Bilayers.

3.4.1 Structural Parameters of Lipid Bilayers Measured by X-ray Diffraction.

3.4.2 Interactions between Bilayers.

3.4.3 Dynamics and Order Parameters of Bilayers Determined by EPR and NMR Spectroscopy and by Optical Spectroscopy Methods.

3.5 Phase Transitions of Lipid Bilayers.

3.5.1 Lyotropic and Thermotropic Transitions.

3.5.2 Thermodynamics of Phase Transitions.

3.5.3 Trans-Gauche Isomerization.

3.5.4 Order Parameter.

3.5.5 Cooperativi…