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Peptide Synthesis and Applications

  • Fester Einband
  • 262 Seiten
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Hands-on experts describe in step-by-step detail the key methodologies of contemporary peptide synthesis and illustrate their nume... Weiterlesen
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Beschreibung

Hands-on experts describe in step-by-step detail the key methodologies of contemporary peptide synthesis and illustrate their numerous applications. The techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included.

Zusammenfassung
Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases.

Inhalt
Part I: Common Strategies Fundamentals of Modern Peptide Synthesis Muriel Amblard, Jean-Alain Fehrentz, Jean Martinez, and Gilles Subra Chimerism: A Strategy to Expand the Utility and Applications of Peptides John Howl Part II: Synthetic Methodologies and Applications Modification of Peptides and Other Drugs Using Lipoamino Acids and Sugars Joanne T. Blanchfield and Istvan Toth Synthesis of Linear, Branched, and Cyclic Peptide Chimera Gábor Mezö and Ferenc Hudecz Synthesis of Cell-Penetrating Peptides for Cargo Delivery Margus Pooga and Ülo Langel Incorporation of the Phosphotyrosyl Mimetic 4(Phosphonodifluoromethyl)phenylalanine (F2Pmp) Into Signal Transduction-Directed Peptides Zhu-Jun Yao, Kyeong Lee, and Terrence R. Burke, Jr. Expressed Protein Ligation for Protein Semisynthesis and Engineering Zuzana Machova and Annette G. Beck-Sickinger Cellular Delivery of Peptide Nucleic Acid by Cell-Penetrating Peptides Kalle Kilk and Ülo Langel Quenched Fluorescent Substrate-Based Peptidase Assays Rebecca A. Lew, Nathalie Tochon-Danguy, Catherine A. Hamilton, Karen M. Stewart, Marie-Isabel Aguilar, and A. Ian Smith A Convenient Method for the Synthesis of Cyclic Peptide Libraries Gregory T. Bourne, Jonathon L. Nielson, Justin F. Coughlan, Paul Darwen, Marc R. Campitelli, Douglas A. Horton, Andreas Rhümann, Stephen G. Love, Tran T. Tran, and Mark L. Smythe High-Throughput Peptide Synthesis Michal Lebl and John Hachmann Backbone Amide Linker Strategies for the Solid-Phase Synthesis of C-Terminal Modified Peptides Jordi Alsina, Steven A. Kates, George Barany, and Fernando Albericio Synthesis of Peptide Bioconjugates Ferenc Hudecz Part III: Practical Guides Protein Identification by Mass Spectrometric Analyses of Peptides AshleyMartin Manual Solid-Phase Synthesis of Glutathione Analogs: A Laboratory-Based Short Course Ursel Soomets, Mihkel Zilmer, and Ülo Langel Index

Produktinformationen

Titel: Peptide Synthesis and Applications
Editor:
EAN: 9781588293176
ISBN: 978-1-58829-317-6
Format: Fester Einband
Herausgeber: Springer, Berlin
Genre: Biologie
Anzahl Seiten: 262
Gewicht: 565g
Größe: H229mm x B229mm x T152mm
Jahr: 2005
Untertitel: Englisch
Auflage: 2005.

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