Peptide Microarrays

This expert volume provides insights into the technological fundamentals together with a comprehensive overview of the potentialities of peptide microarray technology in basic research and clinical assays. Advancements made in recent years in peptide library synthesis, immobilization chemistry and array production have created a foundation from which different new applications are derived, extending the ways in which peptide microarray technology is applied every day. Divided into three sections, the book covers cutting-edge methods and technology, chemoselective strategies for peptide immobilization, and peptide microarrays for medical applications. Written for the high successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls.

Fully updated and authoritative, Peptide Microarrays: Methods and Protocols, Second Edition seeks to encourage scientists to apply current peptide array protocols to the study of interesting new biochemical and medical questions and to assist researchers aiming at developing new methods to further develop peptide microarray technology.

Includes cutting-edge methods and protocols for peptide microarray study Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt
Peptide Arrays on Planar Supports.- High-Throughput Microarray Incubations Using Multi-Well Chambers.- Analysis of Protein Tyrosine Kinase Specificity Using Positional Scanning Peptide Microarrays.- Secondary Structure Determination of Peptides and Proteins After Immobilization.- Peptides and Anti-Peptide Antibodies for Small and Medium Scale Peptide and Anti-Peptide Affinity Microarrays: Antigenic Peptide Selection, Immobilization, and Processing.- Low-Cost Peptide Microarrays for Mapping Continuous Antibody Epitopes.- The Peptide Microarray-Based Resonance Light Scattering Assay for Sensitively Detecting Intracellular Kinase Activity.- Anomalous Reflection of Gold: A Novel Platform for Biochips.- High Throughput Peptide Screening on a Bimodal Imprinting Chip Through MS-SPRi Integration.- Analyzing Peptide Microarray Data with the R pepStat Package.- Chemoselective Strategies to Peptide and Protein Bioprobes Immobilization on Microarray Surfaces.- Manufacturing of Peptide Microarrays Based on Catalyst-Free Click Chemistry.- Clickable Polymeric Coating for Oriented Peptide Immobilization.- Oriented Peptide Immobilization on Microspheres.- A Cell Microarray Format: A Peptide Release System Using a Photo-Cleavable Linker for Cell Toxicity and Cell Uptake Analysis.- Peptide Microarrays for Medical Applications in Autoimmunity, Infection, and Cancer.- Synthetic Peptide-Based ELISA and ELISpot Assay for Identifying Autoantibody Epitopes.- IgE and IgG4 Epitope Mapping of Food Allergens with a Peptide Microarray Immunoassay.- IgE Epitope Mapping Using Peptide Microarray Immunoassay.- Spot Synthesis: An Optimized Microarray to Detect IgE Epitopes.- Mapping of Epitopes Occurring in Bovine s1-Casein Variants by Peptide MicroarrayImmunoassay.