This volume provides the first discussion of orchid protcorm and propagation, detailing genome editing research and offers orchid conservation and ecology. Chapters emphasize both the theory and practice of protocorm manipulation, describing protocorm's biology, and a range of related topics useful in studying protocorm.

Authoritative and cutting-edge, *Orchid Propagation: The Biology and Biotechnology of the Protocorm* aims to be a useful practical guide to researches to help further their study in this field.

Discusses orchid protcorm and propagation Provides orchid genome editing research Offers orchid conservation and ecology

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Inhalt

The orchid protocorm.- Asymbiotic protocorm morphogenesis.- Symbiotic protocorm development.- Protocorm regeneration and protocorm-like bodies.- Protocorm-like body induction and development using in vitro culture techniques and biotechnological methods.- In vitro asymbiotic and symbiotic germination techniques for terrestrial orchid propagation.- Seed/seedling baiting to isolate orchid mycorrhizal fungi for seed germination and seedling growth.- The direct symbiotic germination of orchid seeds in the field.- Dendrobium candidum Wall. ex Lindl.: Production of protocorm-like body (PLB) biomass and bioactive compounds.- Protocorm-like bodies regeneration from callus cultures of Phalaenopsis.- A microtome-based sectioning method for generating thin cell layer cultures in Phalaenopsis.- Orchid cryopreservation via protocorms.- Transcriptomics analysis in the study of mycorrhizal symbiosis in orchids.- CRISPR-Cas9 technology in plant genomics research A review and tutorial for implementation with considerations for orchid genetic studies.- The potential role of endorhizal bacteria associated with Bletilla formosana mycorrhiza during the growth stage.- Histological methods used in the study of protocorm. I. Background information and references.- Histological methods used in the study of protocorm. II. Techniques and protocols.- Visualization of mycorrhizal fungi in orchid protocorms.- Transplant and care for tissue-cultured orchid seedlings.