Oogenesis

This volume presents methods and techniques to study oogenesis in a broad range of organisms, from plants to mammals. Oogenesis: Methods and Protocols guides readers through protocols on models of developmental biology, oogenesis in plants, worms, fruit flies, mosquitos, butterflies, starfish, zebrafish, frog, chicken and mouse. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Oogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Histological Analysis of the Arabidopsis Gynoecium and Ovules Using Chloral Hydrate Clearing and Differential Interference Contrast Light Microscopy.- Indirect Immunofluorescence of Proteins in Oogenic Germ Cells of Caenorhabditis Elegans.- Antibody Staining In Drosophila Germaria.- In Vitro < Culturing and Live Imaging of Drosophila Egg Chambers: A History and Adaptable Method.- Using Fluorescent Reporters to Monitor Autophagy in the Female Germ Line Cells in Drosophila Melanogaster.- Analysis of Phagocytosis in the Drosophila Ovary.- Immuno-Gold Labeling Of Drosophila Follicles For Transmission Electron Microscopy.- Preparation of Drosophila Follicles for Transmission Electron Microscopy.- Quantitative Real-Time PCR Analysis of Gene Transcripts of Mosquito Follicles.- Studying Oogenesis in a Non-Model Organism Using Transcriptomics; Assembling, Annotating, and Analysing your Data.- Live Imaging of Centriole Dynamics by Fluorescently-Tagged Proteins un Starfish Oocyte Meiosis.- High-Pressure Freezing Electron Microscopy of Zebrafish Oocytes.- Cytoskeleton and Cytoskeleton-Bound RNA Visualization in Frog and Insect Oocytes.- RNA FISH, DNA FISH and Chromosome Painting of Chicken Oocytes.- Preparation of Cell Lysate from Mouse Oocytes for Western Blotting Analysis.- Quantitative Microinjection of Morpholino Antisense Oligonucleotides into Mouse Oocytes to Examine Gene Function in Meiosis-I.- Monitoring Calcium Oscillations in Fertilized Mouse Eggs.- Live Imaging of Intracellular Dynamics During Meiotic Maturation in Mouse Oocytes.- Isolation of Mammalian Oogonial Stem Cells by Antibody-Based Fluorescence Activated Cell Sorting.- Immunohistochemistry of Paraffin Sections from Mouse Ovaries.