Michael Potter, Fritz Melchers, Martin Weigert The second workshop on Mechanisms of B Cell Neoplasia was held in Bethesda, Maryland in Wilson Hall at the National Institutes of Health on March 5, 6, and 7, 1984. It followed a workshop on the same topic that was held at the Basel Institute for Immunology, March 15-17, 1983. That first meeting attempted to bring together cell biologists, experimental pathologists and molecular geneti cists interested in B cells, to discuss pathogenetic processes in the development and maintenance of the neoplastic state. The impetus for this discussion emanated from two important developments: first, the discovery of the viral promoter insertion mechanism for acti vating the myc oncogene in bursal lymphomatosis by Hayward, Neil, and Astrin;-second, the findings that the non-random chromosomal trans locations involving the immunoglobulin gene chromosomes occur red in very high frequencies in murine plasmacytomas and human Burkitt's lymphomas. During the planning stages of that meeting Shen-Ong et al. discovered that non-random translocations activated the myc oncogene. Promoter insertions and non-random trans locations were-rwo mechanisms that caused transcription of the myc oncogene messages in three different kinds of well defined experimental and clinical B cell tumors. Unregulated myc gene transcription provided the first evidence of a specific bioChemical lesion in B cell neo plasia.

Inhalt
Cooperation of Oncogenes in B-Cell Neoplasia.- Introductory Remarks.- Activated Oncogenes in Burkitt's Lymphoma.- Detection of an Adenovirus E1A-like Activity in Mammalian Cells.- Does the Large T Protein of Polyoma Virus Regulate the Expression of the Celluar myc Gene?.- Genome Structure and Host Range Restriction of the Lymphotropic Papovavirus (LPV): Identification of a Viral Lymphocyte Specific Enhancer Element.- Tlym-I, a Stage Specific Transforming Gene Shares Homology with MHC I Region Genes.- Structural and Functional Analysis of ras and Blym Oncogenes.- Structural Organization of Mouse emyb Locus and the Mechanism of its Rearrangement in ABPL-2 Tumor Line Induced by Pristane and Abelson Murine Leukemia Virus.- Disruption and Activation of the c-myb Locus by M-MuLV Insertion in Plasmacytoid Lymphosarcomas Induced by Pristane and Abelson Viruses.- Follicular Hyperplasia in the Prelymphomatous Avian Bursa, Relationship to the Incidence of B-Cell Lymphomas.- Growth Factors in B-Cell Neoplasia.- Multiple Steps in the Transformation of Normal B Cells Towards Neoplasia.- Transferrin Receptors Regulate Proliferation of Normal and Malignant B Cells.- Cloned Neoplastic B Cells Release a Growth Factor Which Augments Lymphokine-Mediated Proliferation of Normal B Cells.- The Action of an Lyb2.1-Specific Monoclonal Antibody in Soluble or Immobilized Form on Resting and Activated B Cells.- T Cell Factors Regulating B Cell Activation and Differentiation.- Interleukin 3 Dependent Retrovirus Induced Lymphomas: Loss of the Ability to Terminally Differentiate in Response to Differentiation Factors.- Growth Regulation of a B-Cell Lymphoma via the Antigen Receptor.- Interleukin-2 Receptors on Activated Malignant and Normal B-Cells.- Immuno Affinity Purified Interleukin 2 (IL2)Induces B Cell Growth.- Factors Affecting Normal and Malignant B Lymphocyte Precursors.- Immunoglobulin Heavy Chain Switching in Cultured I.29 Murine B Lymphoma Cells: Commitment to an IgA or IgE Switch.- Regulation of myc Gene Expression in Normal and Neoplastic Cells.- The Regulation of c-myc by Growth Signals.- Immune Regulation of the c-myc Oncogene in a Murine B Lymphoma.- The Translocated c-myc Oncogene of Burkitt Lymphoma is Differentially Regulated in Lymphoblastoid vs Plasma Cells.- Mechanism of myc Gene Activation.- Modes of c-myc Oncogene Activation in Murine Plasmacytomas.- DNA Sequences Involved in the Rearrangement and Expression of the Murine c-myc Gene.- Activation of the c-myc Oncogene in B and T Lymphoid Tumors.- The c-myc Gene Paradox in Burkitt's Lymphoma Chromosomal Translocation.- Amplification, Expression, and Rearrangement of c-myc and N-myc Oncogenes in Human Lung Cancer.- Chromosomal Aberrations in Murine Plasmacytomas.- Chromatin Structural Changes in the Putative Regulatory Region of c-myc Accompany the Translocation in a Burkitt Lymphoma.- Aberrant Rearrangements at the Murine Light Chain Locus in Plasmacytomas.- The myc Gene Product.- myc-Encoded Proteins of Chickens and Men.- Properties of the myc-Gene Product: Nuclear Association, Inhibition of Transcription and Activation in Stimulated Lymphocytes.- In Vitro Culture of B Lymphocytes.- to Long-Term Cultures of Normal B Lymphocytes.- Characteristics of Continuous in vitro Lines of BALB/c and NZB B Cells.- Development of Cells of the B-Lymphocyte Lineage in Longterm Culture.- Coexpression of Lyb-2 and Mac-1by Murine Lymphomas and Subpopulations of Normal Spleen and Bone Marrow Cells.- Ly-1 as a Differentiation Antigen on Normal and Neoplastic B Cells.- Propagation of B Lymphocytes inVitro.- Potential New Systems for the Generation of B Cell Tumors.- Identification of a Transforming Virus from a Lymphoma of a Mouse Infected with a Wild Mouse Retrovirus.- Transformation Capacities of a Murine Retrovirus Encoding an Avian myc Oncogene.- The CH Series of Murine B Cell Lymphomas: Identification of Cross-reactive Idiotypes and Restricted Antigen Specificities.- Interaction of Acute Transforming Retroviruses with Murine Hematopoietic Cells.- Monoclonal Antibody to Abelson Lymphoma Cells.