This book volume provides in depth coverage of the nucleic acid field and aims to represent a broad diversity of the methodologies and a wide range of nucleic-acid-related topics within the fields of molecular biology and biomedicine. Chapters are divided in three sections detailing in vitro detection, nanotechnology and imaging, biomedical applications, and big data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Nucleic Acid Detection and Structural Investigations: Methods and Protocols aims present aims to present methodologies combine cutting-edge innovation with sound theory and practical applications in life sciences.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Optomagnetic Detection of Rolling Circle Amplification Products.- Subtyping of Swine Influenza Using a High-Throughput Real-Time PCR Platform and a Single Microfluidics Device.- RT-qPCR Detection of Low Copy HIV RNA with Yin-Yang Probes.- Solid-phase Hybridization Assay for Detection of Mutated Cancer DNA by Fluorescence.- 5'-Mono- and 5'-bis-pyrene 2'-O-methyl RNA Probes for Detection of RNA Mismatches.- Combined Assay for Detecting Autoantibodies to Nucleic Acids and Apolipoprotein H in Patients with Systemic Lupus Erythematosus.- Electrochemical Detection of dsDNA-specific Antibodies.- Sequence-defined DNA-amphiphiles for Drug Delivery: Synthesis and Self-assembly.- DNA-mediated Liposome Fusion Observed by Fluorescence Spectrometry.- Advanced Fluorescence Imaging to Distinguish Between Intracellular Fractions of Antisense Oligonucleotides.- Methodology for Subcellular Fractionation and microRNA Examination of Mitochondria, Mitochondria Associated ER Membrane (MAM), ER, and Cytosol from Human Brain.- Skeletal Muscle Injury by Electroporation A Model to Study Degeneration/Regeneration Pathways in Muscle.- Isolation and Characterization of Muscle Derived Stem Cells from Dystrophic Mouse Models.- Universal Library Preparation Protocol for Efficient High-Throughput Sequencing Of Double-stranded RNA Viruses.- Quantitation of Molecular Pathway Activation using RNA Sequencing Data.- Molecular Pathway Analysis of Mutation Data for Biomarkers Discovery and Scoring of Target Cancer Drugs.- Oncobox Method for Scoring Efficiencies of Anticancer Drugs Based on Gene Expression Data.- Global Characterization of Circulating Nucleic Acids.