This volume explores the latest advancements in the field of Nuclear Magnetic Resonance (NMR) and discusses how new studies provide insight into the structure, conformation, dynamics, and interactions of glycoproteins. The chapters in this book covers topics such as the preparation of isotope-labeled eukaryotic glycoproteins; applications of paramagnetic NMR to dissect glycosaminoglycan-protein interactions; interactions of mucin glycoproteins; applications of NMR to decipher the interactions between viral proteins and the glycans on host cells; NMR methods to identify biomarkers based on the glycoprotein signals directly acquired from intact biofluids; and the applications of MD simulations and other computational methods to characterize the conformation and dynamics of glycoproteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, NMR of Glycoproteins: Methods and Protocols is a valuable resource for both new and experienced researchers who want to learn more about this important and developing field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

The Glycoworld-Glycoproteins in Nature: A General Overview of the Different Roles that Glycoproteins Exert in Living Organism.- NMR Chemical Shift Prediction of Glycopeptides and Glycoproteins Aided by the Computer Program CASPER.- Chemical Shift Analysis of Oligosaccharides.- Glycoprotein Preparation by Heterologous Expression.- Metabolic 13C-Labeling of IgA Fc Fragment by Transient Mammalian Expression for NMR Analysis.- Investigating Protein-Glycosaminoglycan Interactions using Paramagnetic Glycosaminoglycan Oligosaccharides.- Exploring the Conformational Changes and Dynamics of Mucins in their Free State and in Complex with Mucin-Binding Proteins using NMR.- Virus-Glycan Interactions Studied by Solution NMR.- NMR-Based Quantification of GlycA and GlycB: Advancing Inflammatory Biomarkers in Clinical Diagnostics.- Integration of Experimental and Computational Methods to Characterize Glycoproteins.- Glycoproteins in Viruses.- Towards On-Cell NMR Studies of Glycan-Protein Interactions.