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Nitrogen Fixation

  • Kartonierter Einband
  • 336 Seiten
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This book offers an in-depth overview of the methods that have been applied to studying the nitrogenase at a molecular level, rang... Weiterlesen
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Beschreibung

This book offers an in-depth overview of the methods that have been applied to studying the nitrogenase at a molecular level, ranging from genetic, biochemical, spectroscopic and chemical methods to theoretical calculations.

Few problems in protein biochemistry have proven to be as challenging and recalcitrant as the molecular description of nitrogenase, the catalyst of one of the most remarkable chemical transformations in biological systems: the nucleotide-dependent reduction of atmospheric dinitrogen to bioavailable ammonia. In Nitrogen Fixation: Methods and Protocols, recognized experts in the field provide an up-to-date, in-depth overview of the methods that have been applied to studying the nitrogenase at a molecular level, ranging from genetic, biochemical, spectroscopic, and chemical methods to theoretical calculations. In addition, techniques used to study an enzyme system that is homologous to nitrogenase are described in this book. Written in the highly successful Methods in Molecular Biology™ series format, methods chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and cutting-edge, Nitrogen Fixation: Methods and Protocols will be useful for anyone interested in nitrogenase research and willing to venture further toward addressing the remaining mechanistic and biosynthetic questions of this fascinating enzyme system.

Compiles a detailed collection of techniques to study one of the most difficult problems in protein biochemistry

Features practical, step-by-step methodologies

Includes key tips and expert implementation advice to ensure successful results



Inhalt
Part I: Nitrogen Fixation 1. Historic Overview of Nitrogenase Research Yilin Hu and Markus W. Ribbe 2. Mechanism of Mo-Dependent Nitrogenase Zhi-Yong Yang, Karamatullah Danyal, and Lance C. Seefeldt 3. Assembly of Nitrogenase MoFe Protein Chi-Chung Lee, Aaron W. Fay, Jared A. Wiig, Markus W. Ribbe, and Yilin Hu 4. Genomic Analysis of Nitrogen Fixation Ina P. O'Carroll and Patricia C. Dos Santos 5. Enzymatic Systems with Homology to Nitrogenase Markus J. Bröcker and Jürgen Moser Part II: Genetic and Biochemical Methods 6. Molecular Biology and Genetic Engineering in Nitrogen Fixation Patricia Dos Santos 7. Purification of Nitrogenase Proteins Jared A. Wiig, Chi-Chung Lee, Aaron W. Fay, Yilin Hu, and Markus W. Ribbe 8. Assays of Nitrogenase Reaction Products William E. Newton and Michael J. Dilworth 9. Methods for Nitrogenase-Like Dark Operative Protochlorophyllide Oxidoreductase Markus J. Bröcker and Jürgen Moser Part III: Spectroscopic Methods 10. X-Ray Crystallography Lauren E. Roth and F. Akif Tezcan 11. X-Ray Absorption Spectroscopy Serena DeBeer 12. Small Angle X-Ray Scattering Spectroscopy David W. Mulder and John W. Peters 13. Electron Paramagnetic Resonance Spectroscopy Karamatullah Danyal, Zhi-Yong Yang, and Lance C. Seefeldt 14. Magnetic Circular Dichroism Spectroscopy Brian J. Hales 15. Mössbauer Spectroscopy Boi Hanh Huynh Part IV: Chemical Methods 16. Protocols for Cofactor Isoation of Nitrogenase Aaron W. Fay, Chi-Chung Lee, Jared A. Wiig, Yilin Hu, and Markus W. Ribbe 17. Techniques for Functional and Structural Modeling of Nitrogenase Patrick L. Holland Part V: Theoretical Calculations 18. Nitrogenase Structure and Function Relationships by Density Functional Theory Travis V. Harris and Robert K. Szilagyi 19. Modeling the MoFe Nitrogenase System with Broken Symmetry Density Functional Theory Gregory M. Sandala and Louis Noodleman

Produktinformationen

Titel: Nitrogen Fixation
Untertitel: Methods and Protocols
Editor:
EAN: 9781493958412
ISBN: 1493958410
Format: Kartonierter Einband
Herausgeber: Humana Press
Anzahl Seiten: 336
Gewicht: 634g
Größe: H254mm x B178mm x T18mm
Jahr: 2016
Untertitel: Englisch
Auflage: Softcover reprint of the original 1st ed. 2011

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