Neuropeptide Techniques

Major advances in molecular biology, alternative RNA splicing, protein processing, identification of gene control elements, transgenic animals and bioinformatics open up multiple research avenues and allow for better understanding of neuropeptide production and function. With a selected description of techniques in neuropeptide research, Neuropeptide Methods covers aspects of design, synthesis and biological evaluation of peptides and peptide analogues. Written by leaders in the field, this book explains primary cell cultures and cell lines, as well as techniques in neuropeptide processing, trafficking and secretion. In vivo techniques quantifying blood-brain barrier permeability to small proteins in mice and developmental milestones of behavior in newborn mice are described. Neuropeptide Methods provides the reader with broad perspectives and breadth of knowledge on current topics related to neuropeptide research.

A selected description of techniques in neuropeptide research written by leaders in the field Design synthesis and biological evaluation of peptides and peptide analogues Emphasis to advanced technologies of transfection transgenic delivery and bioinformatics Primary cells and cell lines techniques in neuropeptide processing trafficking and secretion In vivo techniques quantifying blood-brain barrier permeability to small proteins in mice Developmental milestones of behavior in newborn mice

Autorentext
Illana Gozes is a Professor of Clinical Biochemistry, the Lily and Avraham Gildor Chair for the Investigation of Growth Factors, Head of the Dr. Diana and Zelman Elton Elbaum Laboratory for Molecular Neuroendocrinology, and a member of the Sackler Faculty of Medicince at Tel Aviv University in Israel. In addition, she is the Editor in Chief of Humana Press's groundbreaking Journal of Molecular Neuroscience.

Inhalt
A Short Introduction.- Preface.- Contributors.- The Design, Synthesis, and Biological Evaluation of VIP and VIP Analogs David Dangoor, Sara Rubinraut, Mati Fridkin, and Illana Gozes.- Antibody Production: Activity-Dependent Neuroprotective Protein (ADNP) as an Example Illana Gozes, Miri Holtser-Cochav, and Karin Vered.- Primary Cell Cultures and Cell Lines Inna Divinski, Inbar Pilzer, and Illana Gozes.- Transfection of DNA into Cells Inbar Pilzer, Inna Divinski, and Illana Gozes.- Transgenic Delivery and Detection of GFP in Neuropeptide Neurons J.L. Holter, J.S. Davies, P.-S. Man, T. Wells, and D.A. Carter.- Integrative Technologies for Neuropeptide Characterization Sergey E. Ilyin and Carlos R. Plata-Salamán.- Techniques in Neuropeptide Processing, Trafficking, and Secretion Niamh X. Cawley, Tulin Yanik, Irina Arnaoutova,Hong Lou, Nimesh Patel, and Y. Peng Loh.- In vivo Techniques Quantifying BloodBrain Barrier Permeability to Small Proteins in Mice Weihong Pan, and Abba J. Kastin.- Cancer Cell Receptor Internalization and Proliferation: Effects of Neuropeptide Analogs Terry W. Moody, Michael Schumann, and Robert T. Jensen.- Developmental Milestones in the Newborn Mouse Joanna M. Hill, Maria A. Lim, and Madeleine M. Stone.