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Neurodegeneration tackles the challenges involved with neurodegenerative disorders, such as Alzheimer's and Parkinson's disease. The text presents cutting-edge analytical and technological approaches to study the mechanisms underlying neurodegenerative processes.

Neurodegenerative disorders, such as Alzheimer's and Parkinson's disease are a growing burden on our society. Understanding and treating these common diseases is one of the major current challenges for the biomedical field. In Neurodegeneration: Methods and Protocols, experts in the field tackle this challenge and present cutting-edge analytical and technological approaches to study the mechanisms underlying neurodegenerative processes. Organizes into five sections, chapters detail protocols for isolating and culturing cells from the nervous system, production and use of animal models, characterization of cell death, analytical tools to study disease mechanisms, and therapeutic approaches for neurodegeneration. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Neurodegeneration: Methods and Protocols serves as an ideal guide to both novice and professional researchers in the field of neurodegeneration.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras


Part I. Neurodegeneration

1. Introduction to Neurodegenerative Diseases and Related Techniques

Hibiki Kawamata and Giovanni Manfredi

2. In vivo and In vitro Determination of Cell Death Markers in Neurons

Nicole Naniche, Daniela Sua, and Piera Pasinelli

3. Degenerating Processes Identified by Electron Microscopic Immunocytochemical Methods

Teresa A. Milner, Elizabeth M. Waters, Danielle C. Robinson, and Joseph P. Pierce

Part II. Neural Cell Culture Techniques

4. Isolation and Culture of Neurons and Astrocytes from the Mouse Brain Cortex

Hyun-Jeong Kim and Jordi Magrané

5. Isolation and Culture of Postnatal Spinal Motoneurons

Carol Milligan and David Gifondorwa

6. Converting Human Pluripotent Stem Cells to Neural Tissue and Neurons to Model Neurodegeneration

Stuart M. Chambers, Yvonne Mica, Lorenz Studer, and Mark J. Tomishima

7. Neural Differentiation of Induced Pluripotent Stem Cells

Mark Denham and Mirella Dottori

Part III. Models of Neurodegeneration

8. Developing Yeast Models of Human Neurodegenerative Disorders

Alejandro Ocampo and Antoni Barrientos

9. Modeling Dopamine Neuron Degeneration in C. elegans

Michelle L. Tucci, Adam J. Harrington, Guy A. Caldwell, and Kim A. Caldwell

10. Role of the Proteasome in Fly Models of Neurodegeneration

Chun-Hung Yeh, Marlon Jansen, and Thomas Schmidt-Glenewinkel

11. Modeling Neurodegenerative Diseases in Zebrafish Embryos

Angela Laird and Wim Robberecht

12. Practical Considerations of Genetic Rodent Models for Neurodegenerative Diseases

Kindiya Geghman and Chenjian Li

13. Modeling Focal Cerebral Ischemia In vivo

Katherine Jackman, Alexander Kunz, and Costantino Iadecola

14. Pharmacological Models of Parkison's Disease in Rodents

Peter Klivenyi and Laszlo Vecsei

15. Behavioral Phenotyping of Mouse Models of Neurodegeneration

Magali Dumont

Part IV. Neurodegenerative Disease Mechanisms - Proteinopathies

16. Characterization of Amyloid Deposits in Neurodegenerative Diseases

Ruben Vidal and Bernardino Ghetti

17. Detection of Protein Aggregates in Neurodegenerative Diseases

Han-xiang Deng, Eileen H. Bigio, and Teepu Siddique

18. Assessment of Proteasome Impairment and Accumulation/Aggregation of Ubiquitinated Proteins in Neuronal Cultures

Natura Myeku, Maria Jose Metcalfe, Qian Huang, and Maria Figueiredo-Pereira

Part V. Neurodegenerative Disease Mechanisms - Mitochondria

19. Measurements of Threshold of Mitochondrial Transition Pore Opening in Intact and Permeabilized Cells by Flash Photolysis of Caged Calcium

Andrey Y. Abramov and Michael R. Duchen

20. Isolation and Functional Assessment of Mitochondria from Small Amounts of Mouse Brain Tissue

Christos Chinopoulos, Steven F. Zhang, Bobby Thomas, Vadim Ten, and Anatoly A. Starkov

21. Monitoring Mitophagy in Neuronal Cell Cultures

Jianhui Zhu, Ruben K. Dagda, and Charleen T. Chu

Part VI. Neurodegenerative Disease Mechanisms - Biological functions

22. Analysis of Vesicular Trafficking in Primary Neurons by Live Imaging

Davide Tampellini and Gunnar K. Gouras

23. Surface Trafficking of Sodium Channels in Cells and in Hippocampal Slices

Doo Yeon Kim and Dora M. Kovacs

24. Imaging Presynaptic Exocytosis in Corticostriatal Slices

Minerva Yue, David Sulzer, and Nigel Bamford

25. Microscopic Imaging of Intracellular Calcium in Live Cells using Lifetime based Ratiometric Measurements of Oregon Green BAPTA-1

Carli Lattarulo, Diana Thyssen, Kishore V. Kuchibholta, Bradley T. Hyman, and Brian J. Bacskai

26. Electrophysiological Characterization of Neuromuscular Synaptic Dysfunction in Mice

Yoshie Sugiura and Weichun Lin

27. Determination of Neurotransmitter Levels in Models of Parkinson's Disease by HPLC-ECD

Lichuan Yang and Flint M. Beal

28. Designing, Performing, and Interpreting a Microarray-based Gene Expression Study

Giovanni Coppola

Part VII. Therapeutic Approaches in Neurodegeneration

29. Adeno-associated Viral Gene Transfer for Neurodegenerative Diseases

Peter F. Morgenstern, Roberta Marongiu, Sergei A. Musatov, and Michael G. Kaplitt

30. Specific Gene Silencing using RNAi in Cell Culture

Chunxing Yang, Linghua Qiu, and Zuoshang Xu

31. Stem Cell Transplantation for Spinal Cord Neurodegeneration

Angelo C. Lepore and Nicholas J. Maragakis

32. Evaluation of Histone Deacetylase Inhibitors as Therapeutics for Neurodegenerative Diseases

Elisabetta Soragni, Chunping Xu, Andrew Cooper, Heather L. Plasterer, James R. Rusche, and Joel M. Gottesfeld


Titel: Neurodegeneration
Untertitel: Methods and Protocols
EAN: 9781493962204
ISBN: 1493962205
Format: Kartonierter Einband
Herausgeber: Humana Press
Anzahl Seiten: 532
Gewicht: 989g
Größe: H254mm x B178mm x T28mm
Jahr: 2016
Untertitel: Englisch
Auflage: Softcover reprint of the original 1st ed. 2011

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