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Multipotent Stem Cells of the Hair Follicle

  • Fester Einband
  • 180 Seiten
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This volume discusses methods for the study of multipotent and pluripotent stem cells of the hair follicle. The stem cells describ... Weiterlesen
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Beschreibung

This volume discusses methods for the study of multipotent and pluripotent stem cells of the hair follicle. The stem cells described are involved in both the growth of the hair follicle and its production of the hair shaft, as well as the growth of the hair follicle sensory nerve. Multipotent Stem Cells of the Hair Follicle: Methods and Protocols also explores very unexpected results such as that of the hair follicle-associated-pluripotent (HAP) stem cells, which not only have the capability for regenerating the hair follicle sensory nerve, but also can differentiate ex vivo and in vivo to multiple cell types not associated with the hair folliclethese include glial cells, motor neurons, and beating cardiac muscle cells. The potential for HAP stem cells for regenerative medicine is also discussed in detail. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Multipotent Stem Cells of the Hair Follicle: Methods and Protocols is a valuable resource for researchers interested in this field.



Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts



Autorentext
Robert M. Hoffman, Ph.D. Professor of Surgery, University of California, San Diego President, AntiCancer, Inc. San Diego, CA, USA.

Klappentext

This volume discusses methods for the study of multipotent and pluripotent
stem cells of the hair follicle. The stem cells described are involved in both
the growth of the hair follicle and its production of the hair shaft, as well
as the growth of the hair follicle sensory nerve. Multipotent Stem Cells of
the Hair Follicle: Methods and Protocols also explores very unexpected
results such as that of the hair follicle-associated-pluripotent (HAP) stem
cells, which not only have the capability for regenerating the hair follicle
sensory nerve, but also can differentiate ex vivo and in vivo to multiple cell
types not associated with the hair follicle-these include glial cells, motor
neurons, and beating cardiac muscle cells. The potential for HAP stem cells for
regenerative medicine is also discussed in detail. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.

Cutting-edge
and thorough, Multipotent Stem Cells of the Hair Follicle: Methods and
Protocols is a valuable resource for researchers interested in this
field.




Inhalt
1. Introduction to Hair-Follicle-Associated Pluripotent (HAP) Stem CellsRobert M. Hoffman2. Nestin-Based Reporter Transgenic Mouse LinesJohn Mignone, Natalia Peunova, and Grigori Enikolopov3. Discovery of HAP Stem CellsLingna Li and Robert M. Hoffman4. Peripheral-Nerve and Spinal-Cord Regeneration in Mice Using Hair Follicle-Associated-Pluripotent (HAP) Stem CellsYasuyuki Amoh, Kensei Katsuoka, and Robert M. Hoffman5. Construction of Tissue Engineered Nerve Conduits Seeded with Neurons Derived from Hair Follicle Neural Crest Stem CellsFang Liu, Haiyan Lin, and Chuansen Zhang6. Nestin-Expressing Hair Follicle-Associated Pluripotent (HAP) Stem Cells Promote Whisker Sensory-Nerve Growth in Long-Term 3D-Gelfoam HistocultureSumiyuki Mii, Jennifer Duong, Yasunori Tome, Aisada Uchugonova, Fang Liu, Yasuyuki Amoh, Norimitsu Saito, Kensei Katsuoka, and Robert M. Hoffman7. Isolation and Culture of Neural Crest Stem Cells from Human Hair FolliclesRuifeng Yang and Xiaowei Xu8. Isolation of Mouse Hair Follicle Bulge Stem Cells and their Functional Analysis in a Reconstitution AssayYing Zheng, Jen-Chih Hsieh, Julia Escandon, and George Cotsarelis9. Hair Follicle Regeneration by Transplantation of a Bioengineered Hair Follicle GermKatsunari Tezuka, Koh-ei Toyoshima, and Takashi Tsuji10. Hair Induction by Cultured Mesenchymal Cells Using Sphere FormationKazuo Kishi and Ruka Hayashi11. Stereological Quantification of Cell Cycle Kinetics and Mobilization of Epithelial Stem Cells during Wound HealingEduardo Martínez-Martínez, Eileen Uribe-Querol, Claudio I. Galván-Hernández, and Gabriel Gutiérrez-Ospina12. Culture of Dermal Papilla Cells from Ovine Wool Follicles: An In Vitro Model for Papilla Size-DeterminationNicholas W. Rufaut, Allan J. Nixon, and Rodney D. Sinclair13. Isolation and Fluorescence Activated Cell-Sorting of Mouse Keratinocytes Expressing -GalactosidaseMaria Kasper, Rune Toftgård, and Viljar Jaks14. Ectopic Hair Growth from Transplanted Whisker Follicles on the Spinal Cord of MiceWenluo Cao, Fang Liu, Yasuyuki Amoh, and Robert M. Hoffman15. Long-Term Gelfoam® Histoculture of Hair-Shaft-Producing Mouse Whisker FolliclesWenluo Cao, Fang Liu, Yasuyuki Amoh, and Robert M. Hoffman16. From Hair to Heart: Hair Follicle-Associated-Pluripotent (HAP) Stem Cells Differentiate to Beating Cardiac Muscle CellsMasateru Yashiro, Sumiyuki Mii, Ryoichi Aki, Yuko Hamada, Nobuko Arakawa, Katsumasa Kawahara, Robert M. Hoffman, and Yasuyuki Amoh17. Highly Efficient Neural Differentiation of CD34 Positive Hair Follicle-Associated Pluripotent (HAP) Stem Cells Induced by Retinoic Acid and Serum Free MediumMohsen Sagha and Nowruz Najafzadeh18. Cryopreservation of Intact Hair Follicle Maintains Pluripotency of Nestin-Expressing (HAP) Stem CellsSatoshi Kajiura, Sumiyuki Mii, Ryoichi Aki, Yuko Hamada, Nobuko Arakawa, Katsumasa Kawahara, Lingna Li, Kensei Katsuoka, Robert M. Hoffman, and Yasuyuki Amoh

Produktinformationen

Titel: Multipotent Stem Cells of the Hair Follicle
Untertitel: Methods and Protocols
Editor:
EAN: 9781493937844
ISBN: 978-1-4939-3784-4
Format: Fester Einband
Herausgeber: Springer, Berlin
Genre: Biologie
Anzahl Seiten: 180
Größe: H15mm x B261mm x T182mm
Jahr: 2016
Untertitel: Englisch
Auflage: 1st ed. 2016

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