This book details many of the protocols used to study mouse embryology. It provides step-by-step detail essential for reproducible results and contains expert implementation advice.

In Mouse Molecular Embryology: Methods and Protocols , expert researchers in the field detail many of the protocols used to study mouse embryology. These include protocols and techniques that are "close to the embryo": such as, manipulating embryonic gene expression, culturing explanted embryonic tissue and harvesting embryonic RNA. With additional chapters on fluorescence imaging, lineage tracing, and genetic ablation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Mouse Molecular Embryology: Methods and Protocols seeks to aid scientist in the further study of mouse embryo and its relation to other aspects of biological research.

Present cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

In situ Hybridization Methods for Mouse Whole Mounts and Tissue Sections with and Without Additional â-galactosidase Staining.- Two Color In Situ Hybridization of Whole-Mount Mouse Embryos.- Detection and Monitoring of MicroRNA Expression in Developing Mouse Brain and Fixed Brain Cryosections.- Laser Capture Microdissection of Embryonic Cells and Preparation of RNA for Microarray Assays.- EMAGE: Electronic Mouse Atlas of Gene Expression.- Real-time PCR Quantification of gene Expression in Embryonic Mouse Tissue.- Identifying Essential Genes in Mouse Development Via an ENU-based Forward Genetic Approach.- Generation of Mouse Embryos with Small Hairpin RNA-mediated Knockdown of Gene Expression.- Generation of Tissue Organoids by Compaction Reaggregation.- Ultra-Rapid Vitrification of Mouse Oocytes and Embryos.- Mammalian Preimplantation Embryo Culture.- Serum-Free Culture of Mid-Gestation Mouse Embryos: A Tool for the Study of Endoderm-Derived Organs.- Genetically Encoded Probes Provide a Window On Embryonic Arrhythmia.- Microscopic Computed Tomography-based Skeletal Phenotyping for Genetic Model Organisms.- Gene Transfer Techniques in Whole Embryo Cultured Post-Implantation Mouse Embryos.- Segmentation and Quantitative Analysis of Individual Cells in Developmental Tissues.- Protein/Peptide Transduction in Metanephric Explant Culture.- Direction of Cells Programmed to Die in Mouse Embroys.- Microscopic Computed Tomography-based Virtual Histology of Embryos.- Collection and Preparation of Rodent Embryonic Samples for Transcriptome Study.- The Latest Improvements in the Mouse Sperm Preservation.- Analyzing Gene Function in Whole Mouse Embryo and Fetal Organ in vitro .- Using the Textpresso SiteSpecific Recombinases Web Server to Identify Cre Expressing Mouse Strains and Floxed Alleles.- Live Imaging Mouse Embryonic Development: Seeing is Believing and Revealing.- Genetic Cell Ablation .- Essentials of Recombinase-based Genetic Fate Mapping in Mice.