Monoclonal Antibodies: Methods and Protocols examines a collection of state-of-the-art methods that employ monoclonal antibodies in a clinical setting with opening chapters focusing on the gold standard method for generating mouse monoclonal antibodies through hybridoma technology, future methods for engineering recombinant and humanized antibodies, methods for engineering soluble Fc fusion protein, and the use of antibodies and flow cytometry in the quantification of cell signaling proteins.

Specific chapters describe how antibodies are used for the diagnosis and classification of hematologic diseases. Subsequent chapters examine the advantages and most recent advances of using bead-based immunoassays, including the ability of bead-based technology to multiplex and analyze several analytes simultaneously, and the use of beads in detecting fusion proteins resulting from chromosomal translocations. Concluding chapters provide additional examples of methodologies that employ monoclonal antibodies.

Monoclonal Antibodies: Methods and Protocols provides descriptions of methods that cover a wide spectrum of applications in the field of monoclonal antibodies. This field will continue to expand and provide new and innovative techniques, not only in the laboratory, but also as a basis that complements targeted therapy.

Cutting edge methods that employ monoclonal antibodies in a clinical setting In-depth description for generating mouse and recombinant humanized antibodies Comprehensive review of how antibodies are being used in bead-based methods for measuring proteins Reviews of both flow cytometry and immunohistochemistry methods in diagnosis and classification of hematologic diseases

Klappentext

This book examines a collection of state-of-the-art methods that employ monoclonal antibodies in a clinical setting. The chapters offer in-depth description for generating mouse and recombinant humanized antibodies, and a comprehensive review of how antibodies are being used in bead-based methods for measuring proteins. This field will continue to expand and provide new and innovative techniques in the laboratory and as a basis that complements targeted therapy.

Inhalt
Development and Characterization of Mouse Hybridomas.- Recombinant Monoclonal Antibodies.- Soluble Fc Fusion Proteins for Biomedical Research.- The Use of the Antibodies in the Diagnosis of Leukemia and Lymphoma by Flow Cytometry.- Quantification of Surface Antigens and Quantitative Flow Cytometry.- CD13 and CD10 Expression of Granulocytes as Markers for the Functioning of the Immune System.- Monitoring Cell Signaling Pathways by Quantitative Flow Cytometry.- Antibodies and Immunohistochemical Evaluation for the Diagnosis of Hematological Malignancies.- Bead-Based Multianalyte Flow Immunoassays.- Cell-Free Bead-Based Detection of Total and Phosphorylated Proteins in Plasma and Cell Lysates.- Measuring Humanized Antibodies in Plasma of Patients Treated With Antibody-Based Therapy Using Bead-Based Flow Cytometry.- Detection of Chromosome Translocations by Bead-Based Flow Cytometry.- Detection of the MDR1 P-Glycoprotein Expression and Function.- Detection of Human Antibodies Generated Against Therapeutic Antibodies Used in Tumor Therapy.- Measuring Allergen-Specific IgE.