Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols

From probe design to applications in clinical settings, this book provides a diverse set of instructive examples, guided by experts in the field who offer easy-to-follow experimentals.

While originally being used for fluorogenic nucleic acid probes with a stem-loop structure, molecular beacon now refers to a whole family of fluorescence-up probes. This book intends to show a diverse set of instructive examples, from probe design to applications in clinical settings, guided by experts in the field who provide easy-to-follow experiments. The book commences with an introduction to the basic principles of fluorescence, and shows a diverse set of instructive examples, from probe design to applications in clinical settings, molecular beacon technology, the identification of transgenic mice, along with the potential of realtime PCR. This book summarizes all the latest findings and explores powerful techniques designed for geneticist, biotechnologists, molecular and cellular biologists, researchers and developers.

Shows a diverse set of instructive examples from probe design to applications in clinical settings guided by experts in the field who provide easy to follow experiments The book commences with an introduction to the basic principles of fluorescence Extends the potential of realtime PCR Contributions advocate simplifications of the probe technologies Presents a new simplified molecular beacon design EasyBeacons and demonstrates the utility in DNA methylation profiling

Inhalt
Basic Principles of Fluorescence and Energy Transfer.- Applications.- Multiplex Detection of Mutations.- Detection of Salmonella Species in Foodstuffs.- Identification of Homozygous Transgenic Mice by Genomic Real-Time PCR.- Quantitative Analysis of Gene Expression Relative to 18S rRNA in Carcinoma Samples Using the LightCycler® Instrument and a SYBR GreenI-based Assay: Determining FAT10 mRNA Levels in Hepatocellular Carcinoma.- Absolute Quantitation of Feline Leukemia Virus Proviral DNA and Viral RNA Loads by TaqMan® Real-time PCR and RT-PCR.- High-Throughput Real-Time PCR.- Design and Use of Scorpions Fluorescent Signaling Molecules.- Hybridization Probe Pairs and Single-Labeled Probes: an Alternative Approach for Genotyping and Quantification.- New Technologies.- EasyBeacons™ for the Detection of Methylation Status of Single CpG Duplets.- Quenched Autoligation Probes.- HyBeacon® Probes for Rapid DNA Sequence Detection and Allele Discrimination.- FIT-Probes in Real-Time PCR.- SNP Genotyping by Unlabeled Probe Melting Analysis.- Non-Nucleic Acid Targets.- Molecular Beacons for ProteinDNA Interaction Studies.- DNA Polymerase Profiling.- Modular Reporter Hairpin Ribozymes for Analyzing Molecular Interactions.- Screening of Molecular Interactions Using Reporter Hammerhead Ribozymes.