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Cloning, Expression and Characterization of Cyt2Aa1 Toxin

Name: Cloning, Expression and Characterization of Cyt2Aa1 Toxin
Brand: LAP LAMBERT Academic Publishing
SKU: 35656107
Price: 56.8 CHF
Availability: InStock

Von:

Mohamed Abdel Rahman

Michael Palmer

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Beschreibung

A reliable system for expression and purification of the recombinant Cyt2Aa1 toxin has been developed. The recombinant Cyt2Aa1 toxin has been produced, characterized, followed by the construction of the cysteine mutants V186C and L189C by site directed mutagenesis.The hemolytic activity of the V186C mutant exceeds that of wild type Cyt2Aa1 toxin and of the L189C mutant.Calcein release assay experiments have been done to examine the activity of the toxin with different artificial liposomes. It was found that Cyt2Aa1 toxin is very active with DMPC, DMPC+DMPG unilamellar liposomes.

Autorentext

Mohamed Abdel Rahman received his B.S. degree in pharmacy from Al-Azhar University in Egypt in 2001. He participated as a teaching assistant and a researcher at Biology Department in Waterloo University in Canada. In 2010 he received his MSc. in Biology from Waterloo University and continued to study for his Ph.D. from the same university to date.