Mitochondrial Bioenergetics

This second edition volume expands on the previous edition with chapters discussing the latest developments and research initiatives in mitochondrial functions. The chapters in this book explore topics such as high-resolution fluorespirometry and OXPHOS protocols in human cells, analysis of mitochondrial oxygen consumption, mitochondrial bioenergetics, and mitochondrial dynamics in mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Mitochondrial Bioenergetics: Methods and Protocols, Second Edition is a valuable resource for students, and practitioners in the pharmaceutical sciences, environmental sciences, and mitochondrial genetics industries.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Overview of Mitochondrial Bioenergetics.- Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal Cells.- High-Resolution FluoRespirometry and OXPHOS Protocols for Human Cells, Permeabilized Fibres from Small Biopsies of Muscle and Isolated Mitochondria.- High-Throughput Analysis of Mitochondrial Oxygen Consumption.- Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices.- Mitochondrial Membrane Potential () Fluctuations Associated with the Metabolic States of Mitochondria.- Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells.- Comparison of Mitochondrial Incubation Media for Measurement of Respiration and Hydrogen Peroxide Production.- Measurement of Proton Leak in Isolated Mitochondria.- Imaging Mitochondrial Calcium Fluxes with Fluorescent Probes and Single or Two Photon Confocal Microscopy.- Mitochondrial Permeability Transition Pore and Calcium Handling.- Redox Equivalents and Mitochondrial Bioenergetics.- Mitochondrial Bioenergetics by 13 C-NMR Isotopomer Analysis.- Computational Modeling of Mitochondrial Function from a Systems Biology Perspective.- Monitoring the Mitochondrial Dynamics in Mammalian Cells.- Plate-Based Measurement of Superoxide and Hydrogen Peroxide Production by Isolated Mitochondria.- Plate-Based Measurement of Respiration by Isolated Mitochondria.- Detection of Iron Depletion- and Hypoxia-Induced Mitophagy in Mammalian Cells.- The Importance of Calcium Ions for Determining Mitochondrial Glycerol-3-Phosphate Dehydrogenase Activity When Measuring Uncoupling Protein 1 (UCP1) Function in Mitochondria Isolated from Brown Adipose Tissue.- Isolation and Analysis of Mitochondrial Small RNAs from Rat Liver Tissue and HepG2 Cells.- Imaging of Mitochondrial pH Using SNARF-1.- Relation between Mitochondrial Membrane Potential and ROS Formation.- Assessing Spatio-Temporal and Functional Organization of Mitochondrial Networks.- Measurement of Mitochondrial ROS Formation.