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MicroRNAs in Development

  • Fester Einband
  • 200 Seiten
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MicroRNAs in Development explores how to detect and profile miRNA expression, and outlines how to identify and validate miRNA targ... Weiterlesen
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MicroRNAs in Development explores how to detect and profile miRNA expression, and outlines how to identify and validate miRNA targets in animals and plants.

Since the discovery of microRNAs, developmental biologists have striven to understand the role of miRNAs in development and disease. MicroRNAs in Development: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Divided into three convenient sections, this detailed volume covers various techniques to detect and profile miRNA expression, followed by protocols to manipulate the activity of miRNAs in various organisms, and it concludes with a section that outlines different methods to identify and validate miRNA targets in animals and plants. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, MicroRNAs in Development: Methods and Protocols serves as a practical guide for scientists of all backgrounds and conveys the appropriate sense of fascination associated with this vital field of research.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material:

1. In situ Detection of microRNAs in Animals Dylan Sweetman 2. Detection of microRNAs in Plants by In situ Hybridization Éva Várallyay and Zoltán Havelda 3. Detecting Animal and Plant microRNAs by Northern Blotting Sara López-Gomollón 4. Profiling microRNAs by Real-time PCR Nana Jacobsen, Ditte Andreasen, and Peter Mouritzen 5. Detection of Small RNAs and microRNAs using Deep Sequencing Technology Ericka R. Havecker 6. Detection of microRNAs in Prostate Cancer Cells by microRNA Array Xiaoqing Tang, Xiaohu Tang, Jozsef Gal, Natasha Kyprianou, Haining Zhu , and Guiliang Tang 7. microRNA Knock Down by Cholesterol Conjugated Antisense Oligos in Mouse Organ Culture Sharon Kredo-Russo and Eran Hornstein 8. Protocols for Use of Homologous Recombination Gene-targeting to Produce microRNA Mutants in Drosophila Ya-Wen Chen, Ruifen Weng, and Stephen M. Cohen 9. Engineering Elements for Gene Silencing: The Artificial microRNAs Technology Pablo Andrés Manavella and Ignacio Rubio-Somoza 10. Mimicry Technology: Suppressing Small RNA Activity in Plants Ignacio Rubio-Somoza and Pablo Andrés Manavella 11. Experimental Validation of MicroRNA Targets using a Luciferase Reporter System Francisco E. Nicolas 12. Experimental Identification of microRNA Targets by Immunoprecipitation of Argonaute Protein Complexes Michaela Beitzinger and Gunter Meister 13. Comprehensive Identification of miRNA Target Sites in Live Animals Dimitrios G. Zisoulis , Gene W. Yeo, and Amy E. Pasquinelli 14. Target Validation of Plant microRNAs César Llave, José Manuel Franco-Zorrilla, Roberto Solano, and Daniel Barajas 15. A High-throughput Sequencing-based Methodology to Identify All Uncapped and Cleaved RNA Molecules in Eukaryotic Genomes Matthew W. Endres, Rebecca T. Cook, and Brian D. Gregory


Titel: MicroRNAs in Development
Untertitel: Methods and Protocols
EAN: 9781617790829
ISBN: 1617790826
Format: Fester Einband
Herausgeber: Springer-Verlag GmbH
Veröffentlichung: 01.03.2012
Anzahl Seiten: 200
Gewicht: 656g
Größe: H260mm x B183mm x T18mm
Jahr: 2012
Untertitel: Englisch
Auflage: 2011

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