Willkommen, schön sind Sie da!
Logo Ex Libris

Microinjection

  • Fester Einband
  • 214 Seiten
(0) Erste Bewertung abgeben
Bewertungen
(0)
(0)
(0)
(0)
(0)
Alle Bewertungen ansehen
Leseprobe
In this book expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integ... Weiterlesen
CHF 150.00
Print on demand - Exemplar wird für Sie besorgt.
Kein Rückgaberecht!
Bestellung & Lieferung in eine Filiale möglich

Beschreibung

In this book expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function.

As the number of sequenced genomes continues to increase, understanding the functions of newly discovered molecules will require greater efficiency and further study within the context of live cells. In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells. This versatile volume updates established techniques such as cRNA expression in Xenopus oocytes, and examines new, cutting-edge technologies, including antisense morpholino oligonucleotides, RNAi for knockdown experiments, and the use of integrase to produce transgenic animals, all through microinjection techniques that can be easily adopted by any lab. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.

Comprehensive and easily accessible, Microinjection: Methods and Applications is an ideal source for new ideas and techniques certain to aid in the advance of biological research.



Explores a wide variety of model systems, including human, mouse, sea urchin, starfish, zebrafish, and Xenopus oocytes, transformed Caenorhabditis elegans, and tissue culture cells

Contains detailed methods easily adaptable to virtually any system

Covers gene and protein function in living cells, analysis of signal transduction pathways in vivo, single cell analysis, systems biology in a living cell, quantitative analysis of signaling transduction, antisense morpholino oligonucleotide and RNAi analysis, quantitative microinjection, and analysis of Gene Expression, among other topics

Expands the capabilities of cellular and molecular biology laboratories and provides scientists with a valuable mechanistic understanding



Zusammenfassung
In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells.

Inhalt
1. Expression of Exogenous mRNA in Xenopus laevis Embryos for the Study of Cell Cycle Regulation Jill C. Sible and Brian N. Wroble 2. Use of Luciferase Chimaera to Monitor PLC Expression in Mouse Eggs Karl Swann, Karen Campbell, Yuansong Yu, Christopher Saunders, and F. Anthony Lai 3. Analysis of 14-3-3 Family Member Function in Xenopus Embryos by Microinjection of Antisense Morpholino Oligos Jeffrey M. C. Lau and Anthony J. Muslin 4. A Microinjectable Biological System, the Xenopus Oocyte, as an Approach to Understanding Signal Transduction Protein Function Katia Cailliau and Edith Browaeys-Poly 5. Combining Microinjection and Immunoblotting to Analyze MAP Kinase Phosphorylation in Single Starfish Oocytes and Eggs David J. Carroll and Wei Hua 6. Analysis of Signaling Pathways in Zebrafish Development by Microinjection William H. Kinsey 7. Protein Inhibition by Microinjection and RNA-Mediated Interference in Tissue Culture Cells: Complimentary Approaches to Study Protein Function Jane R. Stout, Rania S. Rizk, and Claire E. Walczak 8. DNA Delivery by Microinjection for the Generation of Recombinant Mammalian Cell Lines Sebastien Chenuet, Madiha Derouazi, David Hacker, and Florian Wurm 9. Bacteriophage fC31 Integrase Mediated Transgenesis in Xenopus laevis for Protein Expression at Endogenous Levels Bryan G. Allen and Daniel L. Weeks 10. Germline Transformation of Caenorhabditis elegans by Injection Pavan Kadandale, Indrani Chatterjee, and Andrew Singson 11. Quantitative Microinjection of Mouse Oocytes and Eggs Douglas Kline 12. Microinjection of Follicle-Enclosed Mouse Oocytes Laurinda A. Jaffe, Rachael P. Norris, Marina Freudzon, William J. Ratzan, and Lisa M. Mehlmann 13. Functional Studies of Regulatory Genes in the Sea Urchin Embryo Vincenzo Cavalieri, Maria Di Bernardo, and Giovanni Spinelli 14. Exploring the Cytoskeleton during Intracytoplasmic Sperm Injection in Humans Vanesa Y. Rawe and Héctor Chemes 15. Somatic Cell Nuclear Transfer in the Mouse Satoshi Kishigami and Teruhiko Wakayama

Produktinformationen

Titel: Microinjection
Untertitel: Methods and Applications
Editor:
EAN: 9781588298843
ISBN: 978-1-58829-884-3
Format: Fester Einband
Herausgeber: Springer, Berlin
Genre: Biologie
Anzahl Seiten: 214
Gewicht: 712g
Größe: H263mm x B263mm x T198mm
Jahr: 2008
Auflage: 2009

Weitere Produkte aus der Reihe "Methods in Molecular Biology"

Teil 518
Sie sind hier.