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Microfluidic Techniques

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Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing lar... Weiterlesen
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Beschreibung

Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Inhalt
Overview of Advances in Microfluidics and Microfabrication Shelley D. Minteer and Christine M. Moore Part I. Microfabrication Methods Introduction to Microfabrication Techniques Rabih Zaouk, Benjamin Y. Park, and Marc J. Madou Fabrication of Polydimethylsiloxane Microfluidics Using SU-8 Molds Rabih Zaouk, Benjamin Y. Park, and Marc J. Madou Fabrication of Microelectrodes Using the Lift-Off Technique Benjamin Y. Park, Rabih Zaouk, and Marc J. Madou Laser Ablation as a Fabrication Technique for Microfluidic Devices Emanuel A. Waddell Reversible, Room Temperature Bonding of Glass Devices for Microfluidics Loranelle L. Lockyear Part II. Biological Applications Solid-Phase Extraction in Packed Beds on Glass Microdevices Loranelle L. Lockyear Fabrication of Micromachined Magnetic Particle Separators for Bioseparation in Microfluidic Systems Jin-Woo Choi Hydrogel-Immobilized Antibodies for Microfluidic Immunoassays: Hydrogel Immunoassay Gloria Thomas, Emad M. El-Giar, Laurie E. Locascio, and Michael J. Tarlov Polymerase Chain Reaction in Miniaturized Systems: Big Progress in Little Devices Kalee D. Spitzack and Victor M. Ugaz Polymerase Chain Reaction on Microchips Maria C. Carles and Nikolaus J. Sucher A Sensitive Sandwich DNA Array Using Fluorescent Nanoparticle Probes Xiaojun Zhao, David T. Pierce, and Yanfu Huan Microfluidic Ethanol Biobatteries on a Microchip Shelley D. Minteer and Christine M. Moore Microfluidic Chambers for Cell Migration and Neuroscience Research Anne M. Taylor, Seog Woo Rhee, and Noo Li Jeon Microtextured Polydimethylsiloxane Substrates for Culturing Mesenchymal Stem Cells Erik T. K. Peterson and Ian Papautsky Contraction Study of a Single Cardiac Muscle Cell in a Microfluidic Chip Xiujun (James) Li and Paul C. H. Li Microscale Integrated Sperm Sorter Yaokuang Chung, Xiaoyue Zhu, Wei Gu, Gary D. Smith, and Shuichi Takayama Index

Produktinformationen

Titel: Microfluidic Techniques
Untertitel: Reviews and Protocols
Editor:
EAN: 9781588295170
ISBN: 978-1-58829-517-0
Format: Fester Einband
Herausgeber: Springer, Berlin
Genre: Biologie
Anzahl Seiten: 248
Gewicht: 1g
Größe: H229mm x B229mm x T152mm
Jahr: 2005
Untertitel: Englisch
Auflage: 2006 ed.

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