For molecules that are not directly electroactive, it is necessary to modify a microelectrode by grafting polymeric or enzymatic membranes capable of translating a local concentration into an electrical current, which is a concept referred to as biosensing. In Microelectrode Biosensors , experts in the field cover the topic of electrochemical biosensor technology as applied to the neurosciences. The volume opens with a section addressing the specific issue of manufacturing biosensors that can be implanted in the central nervous system for neurotransmitter detection, and it then continues with sections on recent studies where biosensors have made a difference in bringing a new level of understanding of signaling mechanisms in neuroscience as well as recent developments in biosensor technology that have not yet been applied to implantable microelectrodes but may have great potential. Written for the Neuromethods series, this work contains the kind of detailed descriptions and implementation advice necessary to achieve successful results.

Focused and cutting-edge, Microelectrode Biosensors serves to inspire the wider neuroscience and physiology community to adopt these powerful methods in their own applications in order to move the field forward with the widespread advances that will most likely flow from the adoption of biosensing as part of the standard laboratory toolkit.

Covers, in great detail, electrochemical biosensor technology as applied to the neurosciences Explores the great progress in this field with in-depth methodologies Features tips from the experts, included to ensure optimal results

Zusammenfassung
Covering the processes and potential of biosensors that can be implanted in the central nervous system for neurotransmitter detection, this book offers practical advice on technique, as well as insight on improved approaches and the advances they might bring.

Inhalt

Microelectrode Designs for Oxidase-Based Biosensors.- Microelectrode Array Fabrication and Optimization for Selective Neurochemical Detection.- The Advantage of Microelectrode Technologies for Measurement in Delicate Biological Environments Such as Brain Tissue.- Hexacyanoferrates as Mediators for Microelectrode Biosensors.- Enzyme Immobilization on Microelectrode Biosensors.- Characterization of PolymerEnzyme Composite Biosensors for Brain Monitoring In Vivo.- Monitoring Extracellular Glutamate in the Brain by Microdialysis and Microsensors.- Sub-Second Measurements of Glutamate and Other Neurotransmitter Signaling Using Enzyme-Based Ceramic Microelectrode Arrays.- Regulation of Extracellular Concentrations of D-Serine in the Central Nervous System Revealed by D-Amino Acid Oxidase Microelectrode Biosensors.- Measurement of Purine Release with Microelectrode Biosensors.- Glucose and Lactate Monitoring Across the Rat Sleep-Wake Cycle.- Forebrain Cholinergic Systems and Cognition: New Insights Based on Rapid Detection of Choline Spikes Using Enzyme-Based Biosensors.- Carbon Nanotubes-Based Microelectrode (Bio)sensors.- Label-Free Affinity Biosensors Based on Electrochemical Impedance Spectroscopy.- Aptamer-Based Electrochemical Biosensors for the Detection of Small Molecules and Plasma Proteins.- Electrochemiluminescent Biosensors: Neuroscience Applications.- Molecularly Imprinted Polymers: Promising Advanced Materials for In Vivo Sensing.