Microbial Production of High-Value Products

This edited volume emphasizes how microorganisms have become a reliable pillar of biotechnology. The authors discuss advances in synthetic biology and genetic engineering that have made it possible to reprogram the microbial cellular capabilities. This enables an economically viable production of high-value products at an industrial level.

The first part of the book provides an overview of synthetic biology and genome editing tools for engineering microbial cell factories in modern fermentation. Readers also learn how high-throughput bioprocessing methods are used to recover and purify microbial products.

The remaining parts of this book explore the implementation and challenges of these upstream and downstream processing techniques for manufacturing high-value products. Cost-effectiveness and quality-control are key factors, when discussing the production of low-molecular-weight products, biopharmaceuticals, biopolymers and protein-based nanoparticles.

This book is a valuable resource for biotechnologists both in the industry and in academia.

Focuses on cost, quality, and scale-up of biomanufacturing high-value products in bacteria Highlights advances in fermentation technology and downstream processing to maximize yields and product purity Covers a big variety of microbial products, e.g., biopharmaceuticals, biopolymers and protein-based nanoparticles

Autorentext
Bernd Rehm received his MSc and PhD degrees (microbiology) from the Ruhr-University Bochum, Germany, in 1991 and 1993, respectively. He continued as a postdoc at the Department of Microbiology and Immunology at the University of British Columbia, Canada. From 1996 to 2003, he was a research group leader at the Institute of Molecular Microbiology and Biotechnology at the University of Münster, Germany, where he also completed his habilitation. In 2003 he was appointed as Associate Professor and in 2005 promoted to Full Professor/Chair of Microbiology at Massey University in New Zealand. From 2013 to 2016 he was principal investigator of the Centre of Research Excellence (New Zealand) at the MacDiarmid Institute of Advanced Materials and Nanotechnology. He was recently appointed as director of the Centre for Cell Factories and Biopolymer at Griffith University (Griffith Institute for Drug Discovery, Australia).

He is editor-in-chief and editor of 5 scientific journals as well as an editorial board member of 10 scientific journals and the sole editor of 5 books. He has authored over 200 scientific publications and holds more than 30 patents. His R&D interests are in the microbial production of polymers and their applications. His recent studies focused on the use of engineered microorganisms to produce functionalized nano-/micro-structures for applications in diagnostics, enzyme immobilization, and antigen delivery.

David Wibowo is currently a Bioprocess Scientist in Pharma Services Group at Thermo Fisher Scientific (Australia).

He was previously working as a Research Fellow at Griffith Institute for Drug Discovery, Griffith University (Australia). He is leading one of the new research avenues in the Centre for Cell Factories and Biopolymers within the Institute, focusing on the development of bio-based polyester particles as effective cancer delivery systems for drugs and vaccines. He also establishes collaborative research development in this area nationally and internationally.

Prior to Griffith, David was the Postdoctoral Research Fellow at the Australian Institute for Bioengineering and Nanotechnology, University of Queensland (Australia). In this role, David led efforts aimed at the large-scale production of silica nanocapsules that he co-invented and their utilities in an agricultural sector. He also actively contributed to broaden the applications of the nanocapsules as an emerging nanocarrier of chemotherapeutic drugs, and benchmarked their performances to other systems like liposomes and PLGA nanoparticles.

David received his PhD in Biomedical Engineering from the University of Queensland in 2015. During his PhD, David co-authored a patent, which now covers Australia (granted), Europe, US, and Canada, that describes a sustainable dual-templating platform technology based on mineralizing biosurfactants for making core-shell silica nanoparticles (also called as nanocapsules).

Inhalt
Advancements in Inducer Systems for Recombinant Protein Production in E. Coli .- Microbial Biosynthesis of Straight-Chain Aliphatic Carboxylic Acids.- Microbial Production of Amines and Amino Acids by Fermentation.- Strategies for Improving Biotherapeutic Protein Production in Microbial Cell Factories.- Current Trends and Prospects in Antimicrobial Peptide Bioprocessing.- Bioproduction of Cyclic Disulfide Rich Peptides for Drug Modalities.- Hyaluronic Acid (Hyaluronan).- Polyhydroxyalkanoates (PHA): Microbial Synthesis of Natural Polyesters.- Recent Advances in Poly-(-Glutamic Acid) Production by Microbial Fermentation.- Bioengineering and Bioprocessing of Virus-Like Particle Vaccines in Escherichia Coli .- Functional Inclusion Bodies.- Encapsulin Nanocompartments for Biomanufacturing Applications.- Lumazine Synthase Nanocompartments.