Leading academic and industrial investigators surveys the world of microarray technology, describing in step-by-step detail diverse DNA and protein assays in clinical laboratories using state-of-the-art technologies. The advanced tools and methods described are designed for mRNA expression analysis, SNP analysis, identification, and quantification of proteins, and for studies of protein-protein interactions. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Includes supplementary material: sn.pub/extras

Klappentext
Microarray technology is now well established in laboratories all over the world, both for rapid detection of genetic mutations and, increasingly, for simultaneously analyzing the expression of a large number of proteins. In Microarrays in Clinical Diagnostics, a panel of academic and industrial investigators surveys the new world of microarray technology, describing in step-by-step detail diverse DNA and protein assays in clinical laboratories using state-of-the-art technologies. The advanced tools and methods described are designed for mRNA expression analysis, SNP analysis, identification and quantification of proteins, and for studies of protein-protein interactions. Among the highlights are universal arrays, antibody microarrays, direct labeling approaches, and quality control techniques for protein microarray-based analysis. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and highly practical, Microarrays in Clinical Diagnostics combines a comprehensive collection of cutting-edge technologies with detailed laboratory methods to help clinical laboratory researchers design and perform microarray experiments for DNA and protein analysis, diagnostic testing, and cancer research.

Inhalt
Noncontact Laser Microdissection and Pressure Catapulting.- Applications of the Universal DNA Microarray in Molecular Medicine.- Sensitive Detection of SARS Coronavirus RNA by a Novel Asymmetric Multiplex Nested RT-PCR Amplification Coupled With Oligonucleotide Microarray Hybridization.- Genotyping Single-Nucleotide Polymorphisms by Minisequencing Using Tag Arrays.- Single-Nucleotide Polymorphism and Mutation Identification by the Nanogen Microelectronic Chip Technology.- Molecular Diagnostic Testing for Inherited Thrombophilia Using Invader®.- Controlled Agitation During Hybridization.- Rapid Screening for 31 Mutations and Polymorphisms in the Cystic Fibrosis Transmembrane Conductance Regulator Gene by Luminex® xMAP™ Suspension Array.- Protein Microarray-Based Screening of Antibody Specificity.- Multiplexed Protein Analysis Using Antibody Microarrays and Label-Based Detection.- Allergen Microarrays.- Enhanced Protein Profiling Arrays for Quantitative Measurement of Protein Expression in Multiple Samples.- Multiplexed Cytokine Sandwich Immunoassays Clinical Applications.- Validation and Quality Control of Protein Microarray-Based Analytical Methods.- Tissue Microarrays.